Induction of haptotactic migration of melanoma cells by neutrophil activating protein/interleukin-8

Ji Ming Wang, Giulia Taraboletti, Kouji Matsushima, Jo Van Damme, Alberto Mantovani

Research output: Contribution to journalArticlepeer-review


Natural or recombinant neutrophil activating cytokine (IL-8) induced migration across polycarbonate filters of human A 2058 melanoma cells. Anti-IL-8 antibodies blocked IL-8 induced melanoma cell migration. Checkerboard experiments revealed a gradient-dependent response of A2058 melanoma cells to IL-8. Filters exposed to IL-8 and washed supported melanoma cell migration, thus implying a haptotactic component in the response. The homologous polypeptide platelet factor 4 was inactive. The observation that IL-8 affects melanoma cells emphasizes the need for a comprehensive analysis of the spectrum of action of platelet factor 4-related peptides. The effect of the inflammatory cytokine IL-8 on melanoma cells may be relevant to augmented secondary localization of tumors at sites of inflammation.

Original languageEnglish
Pages (from-to)165-170
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - May 31 1990

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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