Induction of human NK cell-mediated cytotoxicity by CD40 triggering on antigen presenting cells

Rossella Sartorius, Luciana D'Apice, Pasquale Barba, John Guardiola, Angela Santoni, Francesca Velotti, Piergiuseppe De Berardinis

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Engagement of CD40 on antigen presenting cells (APC) is central to the initiation of cell-mediated immune response. Here, we investigated the ability of CD40 ligation on APC to induce NK cell-mediated cytotoxicity in the human system and the mechanism(s) underlying this process. We showed that APC (consisting in adherent peripheral blood mononuclear cells) (PBMC), pre-stimulated with anti-CD40 monoclonal antibodies and co-cultured with autologous non-adherent PBMC for 5-9 days, induced CD3-/CD56+ NK cell-mediated cytotoxicity as well as CD3+/CD56+ T cell-mediated unrestricted cytotoxic activity. The generation of NK cell-mediated cytotoxicity was independent on cell-to-cell contact between CD40-triggered APC and NK cells. Moreover, we found that IL-12 did not play a role in NK cells induction by anti-CD40 priming, while IL-2 and IL-15 did play a role. Our results provide an insight into the mechanism by which NK cells are activated in peripheral blood and useful informations for therapeutic application of anti-CD40 antibodies.

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalCellular Immunology
Volume221
Issue number2
DOIs
Publication statusPublished - Feb 2003

Fingerprint

Antigen-Presenting Cells
Natural Killer Cells
CD40 Antigens
Blood Cells
Interleukin-15
Interleukin-12
Interleukin-2
Ligation
Anti-Idiotypic Antibodies
Monoclonal Antibodies
T-Lymphocytes

Keywords

  • APC
  • CD3
  • CD56
  • IL-15
  • IL-2
  • Magnetic microbeads
  • NK
  • PBMC
  • Tumor immunity
  • Vaccines

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Sartorius, R., D'Apice, L., Barba, P., Guardiola, J., Santoni, A., Velotti, F., & De Berardinis, P. (2003). Induction of human NK cell-mediated cytotoxicity by CD40 triggering on antigen presenting cells. Cellular Immunology, 221(2), 81-88. https://doi.org/10.1016/S0008-8749(03)00060-1

Induction of human NK cell-mediated cytotoxicity by CD40 triggering on antigen presenting cells. / Sartorius, Rossella; D'Apice, Luciana; Barba, Pasquale; Guardiola, John; Santoni, Angela; Velotti, Francesca; De Berardinis, Piergiuseppe.

In: Cellular Immunology, Vol. 221, No. 2, 02.2003, p. 81-88.

Research output: Contribution to journalArticle

Sartorius, R, D'Apice, L, Barba, P, Guardiola, J, Santoni, A, Velotti, F & De Berardinis, P 2003, 'Induction of human NK cell-mediated cytotoxicity by CD40 triggering on antigen presenting cells', Cellular Immunology, vol. 221, no. 2, pp. 81-88. https://doi.org/10.1016/S0008-8749(03)00060-1
Sartorius, Rossella ; D'Apice, Luciana ; Barba, Pasquale ; Guardiola, John ; Santoni, Angela ; Velotti, Francesca ; De Berardinis, Piergiuseppe. / Induction of human NK cell-mediated cytotoxicity by CD40 triggering on antigen presenting cells. In: Cellular Immunology. 2003 ; Vol. 221, No. 2. pp. 81-88.
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