TY - JOUR
T1 - Induction of mixed-function oxidase by chronic treatment with 2,3,7,8-tetrachloro-dibenzo-p-dioxin in female rats
AU - Cantoni, Lavinia
AU - Rizzardini, Milena
AU - Belvedere, Giorgio
AU - Cantoni, Roberto
AU - Fanelli, Roberto
AU - Salmona, Mario
PY - 1981
Y1 - 1981
N2 - The effect of a 45-week treatment with different doses of 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD) (0.01, 0.10 and 1.00 μg/kg/week) was evaluated in female rat liver by determining cytochrome P-450 and b5 content and the activities of the enzymes cytochrome c reductase, aryl hydrocarbon hydroxylase (AHH) and 7-ethoxycoumarin O-deethylase (7-ECD); TCDD content in the liver was also measured. Cytochrome b5 and cytochrome c reductase were unaffected at any of the dose levels and cytochrome P-450 was significantly induced only at the highest TCDD dose, but marked induction of AHH and 7-ECD was apparent when the animals were treated with 0.01 μg/kg/week; a clear dose-response relationship was present in the induction at the 2 lower doses (0.01 and 0.10 μg/kg/week). The amount of TCDD found in lever tissue (1050, 4740 and 30 700 ppt, respectively, for 0.01, 0.10 and 1.00 μg/kg/week) indicated a relatively higher accumulation of this compound at lower doses.
AB - The effect of a 45-week treatment with different doses of 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD) (0.01, 0.10 and 1.00 μg/kg/week) was evaluated in female rat liver by determining cytochrome P-450 and b5 content and the activities of the enzymes cytochrome c reductase, aryl hydrocarbon hydroxylase (AHH) and 7-ethoxycoumarin O-deethylase (7-ECD); TCDD content in the liver was also measured. Cytochrome b5 and cytochrome c reductase were unaffected at any of the dose levels and cytochrome P-450 was significantly induced only at the highest TCDD dose, but marked induction of AHH and 7-ECD was apparent when the animals were treated with 0.01 μg/kg/week; a clear dose-response relationship was present in the induction at the 2 lower doses (0.01 and 0.10 μg/kg/week). The amount of TCDD found in lever tissue (1050, 4740 and 30 700 ppt, respectively, for 0.01, 0.10 and 1.00 μg/kg/week) indicated a relatively higher accumulation of this compound at lower doses.
UR - http://www.scopus.com/inward/record.url?scp=0019348044&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019348044&partnerID=8YFLogxK
U2 - 10.1016/0300-483X(81)90126-8
DO - 10.1016/0300-483X(81)90126-8
M3 - Article
C2 - 7281204
AN - SCOPUS:0019348044
VL - 21
SP - 159
EP - 167
JO - Toxicology
JF - Toxicology
SN - 0300-483X
IS - 2
ER -