Abstract
Differentiation-inducing ability of γ-radiation. N,N-dimethylformamide and their combination has been tested on human rhabdomyosarcoma RMZ-RC2 clone cells. Ionising radiation at 2-5 Gy doses induced a more differentiated morphology, with the appearance of an increased proportion of multinuclear myotube-like cells, and a significant increase in myosin-positive and multinuclear cells. Radiation appeared to act by inducing de novo differentiated elements. N,N-dimethylformamide was able to induce an increased myosin expression, but did not affect multinuclear cell proportion. The combined treatment (ionising radiation and N,N-dimethylformamide) resulted in an additive increase in the proportion of myosin-positive cells, approaching 25-35%, but de novo differentiated elements were not increased above the levels obtained with irradiation alone.
| Original language | English |
|---|---|
| Pages (from-to) | 519-522 |
| Number of pages | 4 |
| Journal | British Journal of Cancer |
| Volume | 65 |
| Issue number | 4 |
| Publication status | Published - 1992 |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
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Induction of myogenic differentiation in human rhabdomyosarcoma cells by ionising radiation, N,N-dimethylformamide and their combination. / Nicoletti, G.; De Giovanni, C.; Landuzzi, L.; Simone, G.; Rocchi, P.; Nanni, P.; Lollini, P. L.
In: British Journal of Cancer, Vol. 65, No. 4, 1992, p. 519-522.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of myogenic differentiation in human rhabdomyosarcoma cells by ionising radiation, N,N-dimethylformamide and their combination
AU - Nicoletti, G.
AU - De Giovanni, C.
AU - Landuzzi, L.
AU - Simone, G.
AU - Rocchi, P.
AU - Nanni, P.
AU - Lollini, P. L.
PY - 1992
Y1 - 1992
N2 - Differentiation-inducing ability of γ-radiation. N,N-dimethylformamide and their combination has been tested on human rhabdomyosarcoma RMZ-RC2 clone cells. Ionising radiation at 2-5 Gy doses induced a more differentiated morphology, with the appearance of an increased proportion of multinuclear myotube-like cells, and a significant increase in myosin-positive and multinuclear cells. Radiation appeared to act by inducing de novo differentiated elements. N,N-dimethylformamide was able to induce an increased myosin expression, but did not affect multinuclear cell proportion. The combined treatment (ionising radiation and N,N-dimethylformamide) resulted in an additive increase in the proportion of myosin-positive cells, approaching 25-35%, but de novo differentiated elements were not increased above the levels obtained with irradiation alone.
AB - Differentiation-inducing ability of γ-radiation. N,N-dimethylformamide and their combination has been tested on human rhabdomyosarcoma RMZ-RC2 clone cells. Ionising radiation at 2-5 Gy doses induced a more differentiated morphology, with the appearance of an increased proportion of multinuclear myotube-like cells, and a significant increase in myosin-positive and multinuclear cells. Radiation appeared to act by inducing de novo differentiated elements. N,N-dimethylformamide was able to induce an increased myosin expression, but did not affect multinuclear cell proportion. The combined treatment (ionising radiation and N,N-dimethylformamide) resulted in an additive increase in the proportion of myosin-positive cells, approaching 25-35%, but de novo differentiated elements were not increased above the levels obtained with irradiation alone.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026608737&partnerID=8YFLogxK
M3 - Article
C2 - 1562460
AN - SCOPUS:0026608737
VL - 65
SP - 519
EP - 522
JO - British Journal of Cancer
JF - British Journal of Cancer
SN - 0007-0920
IS - 4
ER -