Induction of myogenic differentiation in human rhabdomyosarcoma cells by ionising radiation, N,N-dimethylformamide and their combination

G. Nicoletti, C. De Giovanni, L. Landuzzi, G. Simone, P. Rocchi, P. Nanni, P. L. Lollini

Research output: Contribution to journalArticlepeer-review

Abstract

Differentiation-inducing ability of γ-radiation. N,N-dimethylformamide and their combination has been tested on human rhabdomyosarcoma RMZ-RC2 clone cells. Ionising radiation at 2-5 Gy doses induced a more differentiated morphology, with the appearance of an increased proportion of multinuclear myotube-like cells, and a significant increase in myosin-positive and multinuclear cells. Radiation appeared to act by inducing de novo differentiated elements. N,N-dimethylformamide was able to induce an increased myosin expression, but did not affect multinuclear cell proportion. The combined treatment (ionising radiation and N,N-dimethylformamide) resulted in an additive increase in the proportion of myosin-positive cells, approaching 25-35%, but de novo differentiated elements were not increased above the levels obtained with irradiation alone.

Original languageEnglish
Pages (from-to)519-522
Number of pages4
JournalBritish Journal of Cancer
Volume65
Issue number4
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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