Abstract
Monoclonal antibodies against either monomorphic or polymorphic determinants of class I antigen induced in PBMC and highly purified T lymphocytes the nuclear activity of NF-κB/Rel complexes. These included both p50/p50 and p50/p65 dimers, recognized by specific antibodies in EMSA. The induced complexes were detectable in extracts of cells incubated with anti-class I monoclonal antibody (mAb) for 1.5 h; the induction was maximal at 5 h, persistent at 16 h and no longer observed at 40 h. The mAb failed to induce NF-κB/Rel nuclear activity in cells incubated in the presence of 3,4-dichloroisocoumarin, an inhibitor of IκB-α degradation. Together, these results suggest that class I triggering can induce the activity of NF-κB/Rel nuclear activity in peripheral blood T lymphocytes, thereby modulating the expression of genes regulated by these transcription factors.
Original language | English |
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Pages (from-to) | 1-7 |
Number of pages | 7 |
Journal | Tissue Antigens |
Volume | 50 |
Issue number | 1 |
Publication status | Published - 1997 |
Keywords
- HLA class I antigen
- Human peripheral blood T lymphocyte
- NF-κB/Rel transcription factor
ASJC Scopus subject areas
- Immunology
- Cell Biology