Induction of nuclear factor κB/Rel nuclear activity in human peripheral blood T lymphocytes by anti-HLA class I monoclonal antibodies

M. C. Turco, M. F. Romano, A. Lamberti, A. Petrella, R. Bisogni, S. C. Sun, S. Ferrone, P. Bonelli, M. Cerra, S. Venuta

Research output: Contribution to journalArticlepeer-review

Abstract

Monoclonal antibodies against either monomorphic or polymorphic determinants of class I antigen induced in PBMC and highly purified T lymphocytes the nuclear activity of NF-κB/Rel complexes. These included both p50/p50 and p50/p65 dimers, recognized by specific antibodies in EMSA. The induced complexes were detectable in extracts of cells incubated with anti-class I monoclonal antibody (mAb) for 1.5 h; the induction was maximal at 5 h, persistent at 16 h and no longer observed at 40 h. The mAb failed to induce NF-κB/Rel nuclear activity in cells incubated in the presence of 3,4-dichloroisocoumarin, an inhibitor of IκB-α degradation. Together, these results suggest that class I triggering can induce the activity of NF-κB/Rel nuclear activity in peripheral blood T lymphocytes, thereby modulating the expression of genes regulated by these transcription factors.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalTissue Antigens
Volume50
Issue number1
Publication statusPublished - 1997

Keywords

  • HLA class I antigen
  • Human peripheral blood T lymphocyte
  • NF-κB/Rel transcription factor

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

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