Induction of phagocytic behaviour in human epithelial cells by Escherichia coli cytotoxic necrotizing factor type 1

Loredana Falzano, Carla Fiorentini, Gianfranco Donelli, Eric Michel, Christine Kocks, Pascale Cossart, Lucien Cabanié, Eric Oswald, Patrice Boquet

Research output: Contribution to journalArticle

Abstract

Cytotoxic necrotizing factor type 1 (CNF1) from strains of pathogenic Escherichia coli induces in human epithelial HEp-2 cells, a profound reorganization of the actin cytoskeleton into prominent stress fibres and membrane ruffles. We report here that this process is associated with induction of phagocyticlike activity. CNF1-treated cells acquired the ability to ingest latex beads as well as non-invasive bacteria such as Listeria innocua, which were taken as a model system. Uptake of bacteria was similar to pathogen-induced phagocytosis, since L. innocua transformed with DNA coding for the pore-forming toxin listeriolysin O behaved, with respect to intracellular growth, like the invasive, pathogenic species L. monocytogenes. Our results raise the possibility that, in vivo, pathogenic CNF1 -producing E. coli may invade epithelia by this novel induced phagocyticlike mechanism.

Original languageEnglish
Pages (from-to)1247-1254
Number of pages8
JournalMolecular Microbiology
Volume9
Issue number6
Publication statusPublished - Sep 1993

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Fingerprint Dive into the research topics of 'Induction of phagocytic behaviour in human epithelial cells by Escherichia coli cytotoxic necrotizing factor type 1'. Together they form a unique fingerprint.

  • Cite this

    Falzano, L., Fiorentini, C., Donelli, G., Michel, E., Kocks, C., Cossart, P., Cabanié, L., Oswald, E., & Boquet, P. (1993). Induction of phagocytic behaviour in human epithelial cells by Escherichia coli cytotoxic necrotizing factor type 1. Molecular Microbiology, 9(6), 1247-1254.