Induction of the endoplasmic reticulum stress protein GADD153/CHOP by capsaicin in prostate PC-3 cells: A microarray study

Ana María Sánchez, Javier Martínez-Botas, Sophie Malagarie-Cazenave, Nuria Olea, Diana Vara, Miguel Angel Lasunción, Inés Díaz-Laviada

Research output: Contribution to journalArticle

Abstract

The effect of capsaicin, main pungent ingredient of hot chilli peppers, in the gene expression profile of human prostate PC-3 cancer cells has been analyzed using a microarray approach. We identified 10 genes that were down-regulated and five genes that were induced upon capsaicin treatment. The data obtained from microarray analysis were then validated using quantitative real-time PCR assays and Western blot analysis. The most remarkable change was the up-regulation of GADD153/CHOP, an endoplasmic reticulum stress-regulated gene. Activation of GADD153/CHOP protein was corroborated by immunofluorescence and Western blot. We then tested the contribution of GADD153/CHOP to protection against capsaicin-induced cell death using RNA interference. Blockage of GADD153/CHOP expression by small interfering RNA, significantly reduced capsaicin-induced cell death in PC-3 cells. Taken together, these results suggested that capsaicin induces the antiproliferative effect through a mechanism facilitated by ER stress in prostate PC-3 cells.

Original languageEnglish
Pages (from-to)785-791
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume372
Issue number4
DOIs
Publication statusPublished - Aug 8 2008

Keywords

  • Capsaicin
  • DNA microarrays
  • ER stress
  • GADD153/CHOP
  • PC-3 cells
  • Prostate cancer
  • siRNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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    Sánchez, A. M., Martínez-Botas, J., Malagarie-Cazenave, S., Olea, N., Vara, D., Lasunción, M. A., & Díaz-Laviada, I. (2008). Induction of the endoplasmic reticulum stress protein GADD153/CHOP by capsaicin in prostate PC-3 cells: A microarray study. Biochemical and Biophysical Research Communications, 372(4), 785-791. https://doi.org/10.1016/j.bbrc.2008.05.138