Induction of TrkB by retinoic acid mediates biologic responsiveness to BDNF and differentiation of human neuroblastoma cells

David R. Kaplan, Kazue Matsumoto, Enrico Lucarelli, Carol J. Thielet

Research output: Contribution to journalArticle

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Abstract

Retinoic acid (RA) induces the neuronal differentiation of many human neuroblastoma cell lines. In this study, we show that RA treatment of neuroblastoma cells induces the expression of TrkB, the receptor for the neurotrophins BDNF, NT-3, and NT-4/5. BDNF addition to RA-treated SH-SY5Y neuroblastoma cells stimulated the tyrosine phosphorylation of TrkB and neuronal differentiation. RA treatment of KCNR neuroblastoma cells, which constitutively express BDNF mRNA, resulted in the expression of TrkB and differentiation in the absence of added BDNF. Finally, in 15N neuroblastoma cells, which express BDNF mRNA but do not differentiate in response to RA, RA induced only a truncated form of TrkB. 15N cells transfected with full-length TrkB differentiated in the absence of RA. These results indicate that RA induces the neuronal differentiation of neuroblastoma cells by modulating the expression of neurotrophin receptors.

Original languageEnglish
Pages (from-to)321-331
Number of pages11
JournalNeuron
Volume11
Issue number2
DOIs
Publication statusPublished - 1993

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Brain-Derived Neurotrophic Factor
Tretinoin
Neuroblastoma
trkB Receptor
Nerve Growth Factor Receptors
Messenger RNA
Nerve Growth Factors
Tyrosine
Cell Differentiation
Phosphorylation
Cell Line

ASJC Scopus subject areas

  • Neuroscience(all)

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Induction of TrkB by retinoic acid mediates biologic responsiveness to BDNF and differentiation of human neuroblastoma cells. / Kaplan, David R.; Matsumoto, Kazue; Lucarelli, Enrico; Thielet, Carol J.

In: Neuron, Vol. 11, No. 2, 1993, p. 321-331.

Research output: Contribution to journalArticle

Kaplan, David R. ; Matsumoto, Kazue ; Lucarelli, Enrico ; Thielet, Carol J. / Induction of TrkB by retinoic acid mediates biologic responsiveness to BDNF and differentiation of human neuroblastoma cells. In: Neuron. 1993 ; Vol. 11, No. 2. pp. 321-331.
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