Infected chronic ischemic wound topically treated with a multi-strain probiotic formulation: A novel tailored treatment strategy

Salvatore Venosi, Giancarlo Ceccarelli, Massimiliano De Angelis, Luca Laghi, Laura Bianchi, Ombretta Martinelli, Debora Maruca, Eugenio Nelson Cavallari, Fabrizia Toscanella, Paolo Vassalini, Vito Trinchieri, Alessandra Oliva, Gabriella D'Ettorre

Research output: Contribution to journalArticlepeer-review


Background: A wide debate is ongoing regarding the role of cutaneous dysbiosis in the pathogenesis and evolution of difficult-to-treat chronic wounds. Nowadays, probiotic treatment considered as an useful tool to counteract dysbiosis but the evidence in regard to their therapeutic use in the setting of difficult-to-treat cutaneous ulcers is still poor. Aim: clinical report: An 83-year-old woman suffering a critical limb ischemia and an infected difficult-to-treat ulcerated cutaneous lesion of the right leg, was complementary treated with local application of a mixture of probiotic bacteria. Methods: Microbiological and metabolomic analysis were conducted on wound swabs obtained before and after bacteriotherapy. Results: During the treatment course, a progressive healing of the lesion was observed with microbiological resolution of the polymicrobial infection of the wound. Metabolomic analysis showed a significant difference in the local concentration of propionate, 2-hydroxyisovalerate, 2-oxoisocaproate, 2,3-butanediol, putrescine, thymine, and trimethylamine before and after bacteriotherapy. Conclusion: The microbiological and metabolomic results seem to confirm the usefulness of complementary probiotic treatment in difficult-to-treat infected wounds. Further investigations are needed to confirm these preliminary findings.

Original languageEnglish
Article number364
JournalJournal of Translational Medicine
Issue number1
Publication statusPublished - Nov 9 2019


  • Antimicrobial resistance
  • Bacteriotherapy
  • Metabolomic
  • Topical probiotic
  • Wound
  • Wound care
  • Wound healing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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