Infection perturbs Bach2- and Bach1-dependent erythroid lineage ‘choice’ to cause anemia

Hiroki Kato, Ari Itoh-Nakadai, Mitsuyo Matsumoto, Yusho Ishii, Miki Watanabe-Matsui, Masatoshi Ikeda, Risa Ebina-Shibuya, Yuki Sato, Masahiro Kobayashi, Hironari Nishizawa, Katsushi Suzuki, Akihiko Muto, Tohru Fujiwara, Yasuhito Nannya, Luca Malcovati, Mario Cazzola, Seishi Ogawa, Hideo Harigae, Kazuhiko Igarashi

Research output: Contribution to journalArticle

Abstract

Elucidation of how the differentiation of hematopoietic stem and progenitor cells (HSPCs) is reconfigured in response to the environment is critical for understanding the biology and disorder of hematopoiesis. Here we found that the transcription factors (TFs) Bach2 and Bach1 promoted erythropoiesis by regulating heme metabolism in committed erythroid cells to sustain erythroblast maturation and by reinforcing erythroid commitment at the erythro–myeloid bifurcation step. Bach TFs repressed expression of the gene encoding the transcription factor C/EBPβ, as well as that of its target genes encoding molecules important for myelopoiesis and inflammation; they achieved the latter by binding to their regulatory regions also bound by C/EBPβ. Lipopolysaccharide diminished the expression of Bach TFs in progenitor cells and promoted myeloid differentiation. Overexpression of Bach2 in HSPCs promoted erythroid development and inhibited myelopoiesis. Knockdown of BACH1 or BACH2 in human CD34+ HSPCs impaired erythroid differentiation in vitro. Thus, Bach TFs accelerate erythroid commitment by suppressing the myeloid program at steady state. Anemia of inflammation and myelodysplastic syndrome might involve reduced activity of Bach TFs.

Original languageEnglish
Pages (from-to)1059-1070
Number of pages12
JournalNature Immunology
Volume19
Issue number10
DOIs
Publication statusPublished - Oct 1 2018

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Hematopoietic Stem Cells
Anemia
Transcription Factors
Infection
Myelopoiesis
Inflammation
Myeloid Progenitor Cells
Erythroblasts
Erythroid Cells
Erythropoiesis
Nucleic Acid Regulatory Sequences
Myelodysplastic Syndromes
Hematopoiesis
Heme
Lipopolysaccharides
Gene Expression
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Kato, H., Itoh-Nakadai, A., Matsumoto, M., Ishii, Y., Watanabe-Matsui, M., Ikeda, M., ... Igarashi, K. (2018). Infection perturbs Bach2- and Bach1-dependent erythroid lineage ‘choice’ to cause anemia. Nature Immunology, 19(10), 1059-1070. https://doi.org/10.1038/s41590-018-0202-3

Infection perturbs Bach2- and Bach1-dependent erythroid lineage ‘choice’ to cause anemia. / Kato, Hiroki; Itoh-Nakadai, Ari; Matsumoto, Mitsuyo; Ishii, Yusho; Watanabe-Matsui, Miki; Ikeda, Masatoshi; Ebina-Shibuya, Risa; Sato, Yuki; Kobayashi, Masahiro; Nishizawa, Hironari; Suzuki, Katsushi; Muto, Akihiko; Fujiwara, Tohru; Nannya, Yasuhito; Malcovati, Luca; Cazzola, Mario; Ogawa, Seishi; Harigae, Hideo; Igarashi, Kazuhiko.

In: Nature Immunology, Vol. 19, No. 10, 01.10.2018, p. 1059-1070.

Research output: Contribution to journalArticle

Kato, H, Itoh-Nakadai, A, Matsumoto, M, Ishii, Y, Watanabe-Matsui, M, Ikeda, M, Ebina-Shibuya, R, Sato, Y, Kobayashi, M, Nishizawa, H, Suzuki, K, Muto, A, Fujiwara, T, Nannya, Y, Malcovati, L, Cazzola, M, Ogawa, S, Harigae, H & Igarashi, K 2018, 'Infection perturbs Bach2- and Bach1-dependent erythroid lineage ‘choice’ to cause anemia', Nature Immunology, vol. 19, no. 10, pp. 1059-1070. https://doi.org/10.1038/s41590-018-0202-3
Kato H, Itoh-Nakadai A, Matsumoto M, Ishii Y, Watanabe-Matsui M, Ikeda M et al. Infection perturbs Bach2- and Bach1-dependent erythroid lineage ‘choice’ to cause anemia. Nature Immunology. 2018 Oct 1;19(10):1059-1070. https://doi.org/10.1038/s41590-018-0202-3
Kato, Hiroki ; Itoh-Nakadai, Ari ; Matsumoto, Mitsuyo ; Ishii, Yusho ; Watanabe-Matsui, Miki ; Ikeda, Masatoshi ; Ebina-Shibuya, Risa ; Sato, Yuki ; Kobayashi, Masahiro ; Nishizawa, Hironari ; Suzuki, Katsushi ; Muto, Akihiko ; Fujiwara, Tohru ; Nannya, Yasuhito ; Malcovati, Luca ; Cazzola, Mario ; Ogawa, Seishi ; Harigae, Hideo ; Igarashi, Kazuhiko. / Infection perturbs Bach2- and Bach1-dependent erythroid lineage ‘choice’ to cause anemia. In: Nature Immunology. 2018 ; Vol. 19, No. 10. pp. 1059-1070.
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