Inflammation converts human mesoangioblasts into targets of alloreactive immune responses: Implications for allogeneic cell therapy of DMD

Maddalena Noviello, Francesco Saverio Tedesco, Attilio Bondanza, Rossana Tonlorenzi, Maria Rosaria Carbone, Mattia Francesco Maria Gerli, Sarah Marktel, Sara Napolitano, Maria Pia Cicalese, Fabio Ciceri, Giuseppe Peretti, Giulio Cossu, Chiara Bonini

Research output: Contribution to journalArticlepeer-review

Abstract

Stem cell therapy is a promising approach to regenerate healthy tissues starting from a limited amount of self-renewing cells. Immunological rejection of cell therapy products might represent a major limitation. In this study, we investigated the immunological functional profile of mesoangioblasts, vessel-associated myogenic stem cells, currently tested in a phase 1-2a trial, active in our Institute, for the treatment of Duchenne muscular dystrophy. We report that in resting conditions, human mesoangioblasts are poorly immunogenic, inefficient in promoting the expansion of alloreactive T cells and intrinsically resistant to T-cell killing. However, upon exposure to interferon-γ or differentiation into myotubes, mesoangioblasts acquire the ability to promote the expansion of alloreactive T cells and acquire sensitivity to T-cell killing. Resistance of mesoangioblasts to T-cell killing is largely due to the expression of the intracellular serine protease inhibitor-9 and represents a relevant mechanism of stem cell immune evasion.

Original languageEnglish
Pages (from-to)1342-1352
Number of pages11
JournalMolecular Therapy
Volume22
Issue number7
DOIs
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Genetics
  • Drug Discovery
  • Pharmacology
  • Medicine(all)

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