Inflammatory cell burden and phenotype in endomyocardial biopsies with antibody-mediated rejection (AMR): A multicenter pilot study from the AECVP

M. Fedrigo, O. Leone, M. M. Burke, A. Rice, C. Toquet, D. Vernerey, A. C. Frigo, R. Guillemain, S. Pattier, J. Smith, A. Lota, L. Potena, A. Bontadini, C. Ceccarelli, F. Poli, G. Feltrin, G. Gerosa, E. Manzan, G. Thiene, P. BrunevalA. Angelini, J. P Duong Van Huyen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

This multicenter case-controlled pilot study evaluated myocardial inflammatory burden (IB) and phenotype in endomyocardial biopsies (EMBs) with and without pathologic antibody-mediated rejection (pAMR).Sixty-five EMBs from five European heart transplant centers were centrally reviewed as positive (grade 2, n = 28), suspicious (grade 1, n = 7) or negative (n = 30) for pAMR.Absolute counts of total, intravascular (IV) and extravascular (EV) immunophenotyped mononuclear cells were correlated with pAMR grade, capillary C4d deposition, donor specific antibody (DSA) status and acute cellular rejection (ACR).In pAMR+ biopsies, equivalent number of IV CD3+ T lymphocytes (23 ± 4/0.225 mm2) and CD68+ macrophages (21 ± 4/0.225 mm2) were seen.IB and cell phenotype correlated with pAMR grade, C4d positivity and DSA positivity (p <0.0001).High numbers of IV T lymphocytes were associated with low grade ACR (p = 0.002).In late-occurring AMR EV plasma cells occurring in 34% of pAMR+ EMBs were associated with higher IB.The IB in AMR correlated with pAMR+, C4d positivity and DSA positivity.In pAMR+ equivalent numbers of IV T lymphocytes and macrophages were found.The presence of plasma cells was associated with a higher IB and occurrence of pAMR late after transplantation.

Original languageEnglish
Pages (from-to)526-534
Number of pages9
JournalAmerican Journal of Transplantation
Volume15
Issue number2
DOIs
Publication statusPublished - Feb 1 2015

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Multicenter Studies
Phenotype
Biopsy
Antibodies
Plasma Cells
T-Lymphocytes
Macrophages
Transplantation
Transplants

Keywords

  • Biopsy
  • classification systems: ISHLT classification
  • clinical research
  • heart transplantation: cardiology
  • histopathology
  • macrophage
  • monocyte biology: activation
  • pathology
  • practice
  • rejection: antibody-mediated (ABMR)

ASJC Scopus subject areas

  • Transplantation
  • Immunology and Allergy
  • Pharmacology (medical)
  • Medicine(all)

Cite this

Inflammatory cell burden and phenotype in endomyocardial biopsies with antibody-mediated rejection (AMR) : A multicenter pilot study from the AECVP. / Fedrigo, M.; Leone, O.; Burke, M. M.; Rice, A.; Toquet, C.; Vernerey, D.; Frigo, A. C.; Guillemain, R.; Pattier, S.; Smith, J.; Lota, A.; Potena, L.; Bontadini, A.; Ceccarelli, C.; Poli, F.; Feltrin, G.; Gerosa, G.; Manzan, E.; Thiene, G.; Bruneval, P.; Angelini, A.; Van Huyen, J. P Duong.

In: American Journal of Transplantation, Vol. 15, No. 2, 01.02.2015, p. 526-534.

Research output: Contribution to journalArticle

Fedrigo, M, Leone, O, Burke, MM, Rice, A, Toquet, C, Vernerey, D, Frigo, AC, Guillemain, R, Pattier, S, Smith, J, Lota, A, Potena, L, Bontadini, A, Ceccarelli, C, Poli, F, Feltrin, G, Gerosa, G, Manzan, E, Thiene, G, Bruneval, P, Angelini, A & Van Huyen, JPD 2015, 'Inflammatory cell burden and phenotype in endomyocardial biopsies with antibody-mediated rejection (AMR): A multicenter pilot study from the AECVP', American Journal of Transplantation, vol. 15, no. 2, pp. 526-534. https://doi.org/10.1111/ajt.12976
Fedrigo, M. ; Leone, O. ; Burke, M. M. ; Rice, A. ; Toquet, C. ; Vernerey, D. ; Frigo, A. C. ; Guillemain, R. ; Pattier, S. ; Smith, J. ; Lota, A. ; Potena, L. ; Bontadini, A. ; Ceccarelli, C. ; Poli, F. ; Feltrin, G. ; Gerosa, G. ; Manzan, E. ; Thiene, G. ; Bruneval, P. ; Angelini, A. ; Van Huyen, J. P Duong. / Inflammatory cell burden and phenotype in endomyocardial biopsies with antibody-mediated rejection (AMR) : A multicenter pilot study from the AECVP. In: American Journal of Transplantation. 2015 ; Vol. 15, No. 2. pp. 526-534.
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abstract = "This multicenter case-controlled pilot study evaluated myocardial inflammatory burden (IB) and phenotype in endomyocardial biopsies (EMBs) with and without pathologic antibody-mediated rejection (pAMR).Sixty-five EMBs from five European heart transplant centers were centrally reviewed as positive (grade 2, n = 28), suspicious (grade 1, n = 7) or negative (n = 30) for pAMR.Absolute counts of total, intravascular (IV) and extravascular (EV) immunophenotyped mononuclear cells were correlated with pAMR grade, capillary C4d deposition, donor specific antibody (DSA) status and acute cellular rejection (ACR).In pAMR+ biopsies, equivalent number of IV CD3+ T lymphocytes (23 ± 4/0.225 mm2) and CD68+ macrophages (21 ± 4/0.225 mm2) were seen.IB and cell phenotype correlated with pAMR grade, C4d positivity and DSA positivity (p <0.0001).High numbers of IV T lymphocytes were associated with low grade ACR (p = 0.002).In late-occurring AMR EV plasma cells occurring in 34{\%} of pAMR+ EMBs were associated with higher IB.The IB in AMR correlated with pAMR+, C4d positivity and DSA positivity.In pAMR+ equivalent numbers of IV T lymphocytes and macrophages were found.The presence of plasma cells was associated with a higher IB and occurrence of pAMR late after transplantation.",
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AU - Vernerey, D.

AU - Frigo, A. C.

AU - Guillemain, R.

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AU - Smith, J.

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AU - Gerosa, G.

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