Inflammatory cells, cytokines and matrix metalloproteinases in amicrobial pustulosis of the folds and other neutrophilic dermatoses

A. V. Marzano, Massimo Cugno, V. Trevisan, R. Lazzari, D. Fanoni, E. Berti, C. Crosti

Research output: Contribution to journalArticle

Abstract

Amicrobial pustulosis of the folds (APF) is a rare cutaneous disease characterized by relapsing sterile pustules frequently associated with autoimmune disorders. Although APF pathophysiology is still undefined, scattered reports suggest involvement of neutrophils. The aim of the present study is to evaluate the role of the skin inflammatory infiltrate, selected multifunctional cytokines and effectors of tissue damage in APF and other neutrophilic dermatoses. We studied, by immunohistochemical methods, inflammatory cell markers (CD3, CD163, myeloperoxidase), cytokines (TNF-α, IL-8, IL-17), metalloproteinases (MMP-2, MMP-9) and vascular-endothelial-growth-factor (VEGF) in lesional skin from six patients with APF, 11 with pyoderma gangrenosum (PG), 7 with Sweet's syndrome, and in 20 normal skin samples. Immunoreactivities of CD3, CD163, myeloperoxidase, TNF-α, IL-8, IL-17, MMP-2, MMP-9 and VEGF were significantly higher in APF, PG and Sweet's syndrome than in controls (p=0.0001). IL-8 was more expressed in PG than in APF (P=0.002) and Sweet's syndrome (p=0.001). In APF, MMP-9 reactivity was higher than in Sweet's syndrome (p=0.035), but less intense than in PG (p=0.020). Our study supports the role of proinflammatory cytokines/chemokines and MMPs as important effectors for the tissue damage in APF similarly to classic neutrophilic dermatoses.

Original languageEnglish
Pages (from-to)451-460
Number of pages10
JournalInternational Journal of Immunopathology and Pharmacology
Volume24
Issue number2
Publication statusPublished - Apr 2011

Keywords

  • Amicrobial pustulosis of the folds
  • Cytokines
  • Metalloproteinases
  • Neutrophilic dermatoses
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy

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