Inflammatory cytokines provide a third signal for activation of naive CD4+ and CD8+ T cells

Julie M. Curtsinger, Clint S. Schmidt, Anna Mondino, Debra C. Lins, Ross M. Kedl, Marc K. Jenkins, Matthew F. Mescher

Research output: Contribution to journalArticle

Abstract

The effects of inflammatory cytokines on naive T cells have been studied using MHC protein/peptide complexes on microspheres, thus avoiding the use of APCs whose functions may be affected by the cytokines. IL-1, but not IL-12, increased proliferation of CD4+ T cells in response to Ag and IL-2, which is consistent with effects on in vivo priming of CD4+ cells. In contrast, proliferation of CD8+ T cells to Ag and IL-2 required IL-12, and IL-12 replaced adjuvant in stimulating an in vivo response to peptide. These results support a model in which distinct inflammatory cytokines act directly on naive CD4+ and CD8+ T cells to provide a third signal, along with Ag and IL-2, to optimally activate differentiation and clonal expansion.

Original languageEnglish
Pages (from-to)3256-3262
Number of pages7
JournalJournal of Immunology
Volume162
Issue number6
Publication statusPublished - Mar 15 1999

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Interleukin-12
Cytokines
Interleukin-2
T-Lymphocytes
Peptides
Interleukin-1
Microspheres
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Curtsinger, J. M., Schmidt, C. S., Mondino, A., Lins, D. C., Kedl, R. M., Jenkins, M. K., & Mescher, M. F. (1999). Inflammatory cytokines provide a third signal for activation of naive CD4+ and CD8+ T cells. Journal of Immunology, 162(6), 3256-3262.

Inflammatory cytokines provide a third signal for activation of naive CD4+ and CD8+ T cells. / Curtsinger, Julie M.; Schmidt, Clint S.; Mondino, Anna; Lins, Debra C.; Kedl, Ross M.; Jenkins, Marc K.; Mescher, Matthew F.

In: Journal of Immunology, Vol. 162, No. 6, 15.03.1999, p. 3256-3262.

Research output: Contribution to journalArticle

Curtsinger, JM, Schmidt, CS, Mondino, A, Lins, DC, Kedl, RM, Jenkins, MK & Mescher, MF 1999, 'Inflammatory cytokines provide a third signal for activation of naive CD4+ and CD8+ T cells', Journal of Immunology, vol. 162, no. 6, pp. 3256-3262.
Curtsinger JM, Schmidt CS, Mondino A, Lins DC, Kedl RM, Jenkins MK et al. Inflammatory cytokines provide a third signal for activation of naive CD4+ and CD8+ T cells. Journal of Immunology. 1999 Mar 15;162(6):3256-3262.
Curtsinger, Julie M. ; Schmidt, Clint S. ; Mondino, Anna ; Lins, Debra C. ; Kedl, Ross M. ; Jenkins, Marc K. ; Mescher, Matthew F. / Inflammatory cytokines provide a third signal for activation of naive CD4+ and CD8+ T cells. In: Journal of Immunology. 1999 ; Vol. 162, No. 6. pp. 3256-3262.
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