Abstract
The effects of inflammatory cytokines on naive T cells have been studied using MHC protein/peptide complexes on microspheres, thus avoiding the use of APCs whose functions may be affected by the cytokines. IL-1, but not IL-12, increased proliferation of CD4+ T cells in response to Ag and IL-2, which is consistent with effects on in vivo priming of CD4+ cells. In contrast, proliferation of CD8+ T cells to Ag and IL-2 required IL-12, and IL-12 replaced adjuvant in stimulating an in vivo response to peptide. These results support a model in which distinct inflammatory cytokines act directly on naive CD4+ and CD8+ T cells to provide a third signal, along with Ag and IL-2, to optimally activate differentiation and clonal expansion.
| Original language | English |
|---|---|
| Pages (from-to) | 3256-3262 |
| Number of pages | 7 |
| Journal | Journal of Immunology |
| Volume | 162 |
| Issue number | 6 |
| Publication status | Published - Mar 15 1999 |
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ASJC Scopus subject areas
- Immunology
Cite this
Inflammatory cytokines provide a third signal for activation of naive CD4+ and CD8+ T cells. / Curtsinger, Julie M.; Schmidt, Clint S.; Mondino, Anna; Lins, Debra C.; Kedl, Ross M.; Jenkins, Marc K.; Mescher, Matthew F.
In: Journal of Immunology, Vol. 162, No. 6, 15.03.1999, p. 3256-3262.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inflammatory cytokines provide a third signal for activation of naive CD4+ and CD8+ T cells
AU - Curtsinger, Julie M.
AU - Schmidt, Clint S.
AU - Mondino, Anna
AU - Lins, Debra C.
AU - Kedl, Ross M.
AU - Jenkins, Marc K.
AU - Mescher, Matthew F.
PY - 1999/3/15
Y1 - 1999/3/15
N2 - The effects of inflammatory cytokines on naive T cells have been studied using MHC protein/peptide complexes on microspheres, thus avoiding the use of APCs whose functions may be affected by the cytokines. IL-1, but not IL-12, increased proliferation of CD4+ T cells in response to Ag and IL-2, which is consistent with effects on in vivo priming of CD4+ cells. In contrast, proliferation of CD8+ T cells to Ag and IL-2 required IL-12, and IL-12 replaced adjuvant in stimulating an in vivo response to peptide. These results support a model in which distinct inflammatory cytokines act directly on naive CD4+ and CD8+ T cells to provide a third signal, along with Ag and IL-2, to optimally activate differentiation and clonal expansion.
AB - The effects of inflammatory cytokines on naive T cells have been studied using MHC protein/peptide complexes on microspheres, thus avoiding the use of APCs whose functions may be affected by the cytokines. IL-1, but not IL-12, increased proliferation of CD4+ T cells in response to Ag and IL-2, which is consistent with effects on in vivo priming of CD4+ cells. In contrast, proliferation of CD8+ T cells to Ag and IL-2 required IL-12, and IL-12 replaced adjuvant in stimulating an in vivo response to peptide. These results support a model in which distinct inflammatory cytokines act directly on naive CD4+ and CD8+ T cells to provide a third signal, along with Ag and IL-2, to optimally activate differentiation and clonal expansion.
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UR - http://www.scopus.com/inward/citedby.url?scp=0033559126&partnerID=8YFLogxK
M3 - Article
C2 - 10092777
AN - SCOPUS:0033559126
VL - 162
SP - 3256
EP - 3262
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 6
ER -