TY - JOUR
T1 - Inflammatory response and glia activation in developing rat hippocampus after status epilepticus
AU - Ravizza, Teresa
AU - Rizzi, Massimo
AU - Perego, Carlo
AU - Richichi, Cristina
AU - Velískǒvá, Jana
AU - Moshé, Solomon L.
AU - De Simoni, M. Grazia
AU - Vezzani, Annamaria
PY - 2005/7
Y1 - 2005/7
N2 - Purpose: We investigated the activation of microglia and astrocytes, induction of cytokines, and hippocampal neuronal damage, 4 and 24 h after kainic acid-induced status epilepticus (SE) in postnatal day (PN) 9, 15, and 21 rats. Methods: Limbic seizures were induced by systemic injection of kainic acid. Glia activation and neuronal cell loss were studied by using immunocytochemistry and Western blot. Cytokine expression was analyzed by reverse transcriptase- polymerase chain reaction (RT-PCR) followed by Southern blot quantification. Results: After SE onset, hippocampal glia activation, cytokine expression, and neuronal damage are all age-dependent phenomena. In the hippocampus, neuronal injury occurs only when cytokines are induced in glia, and cytokine synthesis precedes the appearance of degenerating neurons. Neuronal injury is more pronounced when interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) are produced in addition to IL-1β. Conclusions: This study shows that cytokine induction in rat brain after sustained seizures is age dependent, and it is associated with the appearance of cell injury.
AB - Purpose: We investigated the activation of microglia and astrocytes, induction of cytokines, and hippocampal neuronal damage, 4 and 24 h after kainic acid-induced status epilepticus (SE) in postnatal day (PN) 9, 15, and 21 rats. Methods: Limbic seizures were induced by systemic injection of kainic acid. Glia activation and neuronal cell loss were studied by using immunocytochemistry and Western blot. Cytokine expression was analyzed by reverse transcriptase- polymerase chain reaction (RT-PCR) followed by Southern blot quantification. Results: After SE onset, hippocampal glia activation, cytokine expression, and neuronal damage are all age-dependent phenomena. In the hippocampus, neuronal injury occurs only when cytokines are induced in glia, and cytokine synthesis precedes the appearance of degenerating neurons. Neuronal injury is more pronounced when interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) are produced in addition to IL-1β. Conclusions: This study shows that cytokine induction in rat brain after sustained seizures is age dependent, and it is associated with the appearance of cell injury.
KW - Brain development
KW - Hippocamus
KW - Interleukins
KW - Neurodegeneration
KW - Rat
KW - Seizures
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U2 - 10.1111/j.1528-1167.2005.01006.x
DO - 10.1111/j.1528-1167.2005.01006.x
M3 - Article
C2 - 15987264
AN - SCOPUS:21844464283
VL - 46
SP - 113
EP - 117
JO - Epilepsia
JF - Epilepsia
SN - 0013-9580
IS - SUPPL. 5
ER -