Influence of eNOS gene polymorphisms on carotid atherosclerosis

C. Fatini, F. Sofi, F. Gensini, E. Sticcini, B. Lari, G. Pratesi, R. Pulli, W. Dorigo, C. Pratesi, G. F. Gensini, R. Abbare

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Introduction. Nitric oxide (NO) is an endothelium-derived relaxing factor which plays a role in atherogenetic events. Polymorphisms in the endothelial NO synthase gene (eNOS) influences the functional activity of the enzyme and affect the susceptibility to atherogenesis. In this study we determined whether T-786C, G894T and 4a/4b eNOS genetic variants may increase the susceptibility to carotid atherosclerosis. Methods. The study groups included 304 consecutive patients with severe carotid stenosis (≥70%) and 544 control subjects. The eNOS polymorphisms were analysed by molecular biology techniques. Results. The genotype distribution and allele frequency of eNOS 4a/4b, but not T-786C and G894T, polymorphism was significantly different between patients and controls. Using logistic regression with adjustment for other risk factors, the 4a allele and the combined genotype 4a4a + 4a4b/894TT + GT and -786CC + TC/894TT + GT were associated with carotid stenosis (OR = 1.5, p = 0.02; OR = 1.8, p = 0.01; OR = 1.5, p = 0.04, respectively). In a subset of patients (30 of 304) with no traditional risk factors for atherosclerosis, a relatively high incidence of the 4a allele and 4a4a + 4a4b/-786CC + TC combined genotype was noted. Discussion. Our findings suggest that the 4a allele and the eNOS combined genotypes are independent predisposing factors to carotid atherosclerosis.

Original languageEnglish
Pages (from-to)540-544
Number of pages5
JournalEuropean Journal of Vascular and Endovascular Surgery
Volume27
Issue number5
DOIs
Publication statusPublished - May 2004

Fingerprint

Carotid Artery Diseases
Nitric Oxide Synthase
Genotype
Genes
Carotid Stenosis
Alleles
Atherosclerosis
Endothelium-Dependent Relaxing Factors
Nitric Oxide Synthase Type III
Gene Frequency
Causality
Molecular Biology
Nitric Oxide
Logistic Models
Incidence
Enzymes

Keywords

  • Carotid atherosclerosis
  • eNOS gene polymorphisms
  • Vascular surgery

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Radiology Nuclear Medicine and imaging
  • Surgery

Cite this

Influence of eNOS gene polymorphisms on carotid atherosclerosis. / Fatini, C.; Sofi, F.; Gensini, F.; Sticcini, E.; Lari, B.; Pratesi, G.; Pulli, R.; Dorigo, W.; Pratesi, C.; Gensini, G. F.; Abbare, R.

In: European Journal of Vascular and Endovascular Surgery, Vol. 27, No. 5, 05.2004, p. 540-544.

Research output: Contribution to journalArticle

Fatini, C, Sofi, F, Gensini, F, Sticcini, E, Lari, B, Pratesi, G, Pulli, R, Dorigo, W, Pratesi, C, Gensini, GF & Abbare, R 2004, 'Influence of eNOS gene polymorphisms on carotid atherosclerosis', European Journal of Vascular and Endovascular Surgery, vol. 27, no. 5, pp. 540-544. https://doi.org/10.1016/j.ejvs.2004.02.008
Fatini, C. ; Sofi, F. ; Gensini, F. ; Sticcini, E. ; Lari, B. ; Pratesi, G. ; Pulli, R. ; Dorigo, W. ; Pratesi, C. ; Gensini, G. F. ; Abbare, R. / Influence of eNOS gene polymorphisms on carotid atherosclerosis. In: European Journal of Vascular and Endovascular Surgery. 2004 ; Vol. 27, No. 5. pp. 540-544.
@article{521fb289f6f2493dae22009fb257eb93,
title = "Influence of eNOS gene polymorphisms on carotid atherosclerosis",
abstract = "Introduction. Nitric oxide (NO) is an endothelium-derived relaxing factor which plays a role in atherogenetic events. Polymorphisms in the endothelial NO synthase gene (eNOS) influences the functional activity of the enzyme and affect the susceptibility to atherogenesis. In this study we determined whether T-786C, G894T and 4a/4b eNOS genetic variants may increase the susceptibility to carotid atherosclerosis. Methods. The study groups included 304 consecutive patients with severe carotid stenosis (≥70{\%}) and 544 control subjects. The eNOS polymorphisms were analysed by molecular biology techniques. Results. The genotype distribution and allele frequency of eNOS 4a/4b, but not T-786C and G894T, polymorphism was significantly different between patients and controls. Using logistic regression with adjustment for other risk factors, the 4a allele and the combined genotype 4a4a + 4a4b/894TT + GT and -786CC + TC/894TT + GT were associated with carotid stenosis (OR = 1.5, p = 0.02; OR = 1.8, p = 0.01; OR = 1.5, p = 0.04, respectively). In a subset of patients (30 of 304) with no traditional risk factors for atherosclerosis, a relatively high incidence of the 4a allele and 4a4a + 4a4b/-786CC + TC combined genotype was noted. Discussion. Our findings suggest that the 4a allele and the eNOS combined genotypes are independent predisposing factors to carotid atherosclerosis.",
keywords = "Carotid atherosclerosis, eNOS gene polymorphisms, Vascular surgery",
author = "C. Fatini and F. Sofi and F. Gensini and E. Sticcini and B. Lari and G. Pratesi and R. Pulli and W. Dorigo and C. Pratesi and Gensini, {G. F.} and R. Abbare",
year = "2004",
month = "5",
doi = "10.1016/j.ejvs.2004.02.008",
language = "English",
volume = "27",
pages = "540--544",
journal = "European Journal of Vascular and Endovascular Surgery",
issn = "1078-5884",
publisher = "W.B. Saunders Ltd",
number = "5",

}

TY - JOUR

T1 - Influence of eNOS gene polymorphisms on carotid atherosclerosis

AU - Fatini, C.

AU - Sofi, F.

AU - Gensini, F.

AU - Sticcini, E.

AU - Lari, B.

AU - Pratesi, G.

AU - Pulli, R.

AU - Dorigo, W.

AU - Pratesi, C.

AU - Gensini, G. F.

AU - Abbare, R.

PY - 2004/5

Y1 - 2004/5

N2 - Introduction. Nitric oxide (NO) is an endothelium-derived relaxing factor which plays a role in atherogenetic events. Polymorphisms in the endothelial NO synthase gene (eNOS) influences the functional activity of the enzyme and affect the susceptibility to atherogenesis. In this study we determined whether T-786C, G894T and 4a/4b eNOS genetic variants may increase the susceptibility to carotid atherosclerosis. Methods. The study groups included 304 consecutive patients with severe carotid stenosis (≥70%) and 544 control subjects. The eNOS polymorphisms were analysed by molecular biology techniques. Results. The genotype distribution and allele frequency of eNOS 4a/4b, but not T-786C and G894T, polymorphism was significantly different between patients and controls. Using logistic regression with adjustment for other risk factors, the 4a allele and the combined genotype 4a4a + 4a4b/894TT + GT and -786CC + TC/894TT + GT were associated with carotid stenosis (OR = 1.5, p = 0.02; OR = 1.8, p = 0.01; OR = 1.5, p = 0.04, respectively). In a subset of patients (30 of 304) with no traditional risk factors for atherosclerosis, a relatively high incidence of the 4a allele and 4a4a + 4a4b/-786CC + TC combined genotype was noted. Discussion. Our findings suggest that the 4a allele and the eNOS combined genotypes are independent predisposing factors to carotid atherosclerosis.

AB - Introduction. Nitric oxide (NO) is an endothelium-derived relaxing factor which plays a role in atherogenetic events. Polymorphisms in the endothelial NO synthase gene (eNOS) influences the functional activity of the enzyme and affect the susceptibility to atherogenesis. In this study we determined whether T-786C, G894T and 4a/4b eNOS genetic variants may increase the susceptibility to carotid atherosclerosis. Methods. The study groups included 304 consecutive patients with severe carotid stenosis (≥70%) and 544 control subjects. The eNOS polymorphisms were analysed by molecular biology techniques. Results. The genotype distribution and allele frequency of eNOS 4a/4b, but not T-786C and G894T, polymorphism was significantly different between patients and controls. Using logistic regression with adjustment for other risk factors, the 4a allele and the combined genotype 4a4a + 4a4b/894TT + GT and -786CC + TC/894TT + GT were associated with carotid stenosis (OR = 1.5, p = 0.02; OR = 1.8, p = 0.01; OR = 1.5, p = 0.04, respectively). In a subset of patients (30 of 304) with no traditional risk factors for atherosclerosis, a relatively high incidence of the 4a allele and 4a4a + 4a4b/-786CC + TC combined genotype was noted. Discussion. Our findings suggest that the 4a allele and the eNOS combined genotypes are independent predisposing factors to carotid atherosclerosis.

KW - Carotid atherosclerosis

KW - eNOS gene polymorphisms

KW - Vascular surgery

UR - http://www.scopus.com/inward/record.url?scp=11144355969&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11144355969&partnerID=8YFLogxK

U2 - 10.1016/j.ejvs.2004.02.008

DO - 10.1016/j.ejvs.2004.02.008

M3 - Article

VL - 27

SP - 540

EP - 544

JO - European Journal of Vascular and Endovascular Surgery

JF - European Journal of Vascular and Endovascular Surgery

SN - 1078-5884

IS - 5

ER -