Influence of f-MLP, ACTH(1-24) and CRH on in vitro chemotaxis of monocytes from centenarians

Susanna Genedani, Monica Filaferro, Chiara Carone, Rita Ostan, Laura Bucci, Elisa Cevenini, Claudio Franceschi, Daniela Monti

Research output: Contribution to journalArticle

Abstract

Objective: The lifelong exposure to a variety of stressors activates a plethora of defense mechanisms, including the hypothalamic-pituitary-adrenal axis which releases neuropeptides affecting the immune responses. Here, we report data on the capability of monocytes from young subjects and centenarians to migrate towards chemotactic stimuli (formyl-methionyl-leucyl-phenylalanine, f-MLP; adrenocorticotropic hormone, ACTH, and corticotrophin-releasing hormone, CRH). Plasma levels of ACTH, CRH and cortisol were measured as an index of ongoing stress response. Methods: Monocyte chemotaxis towards f-MLP (10 -8M), ACTH(1-24) (10-14 and 10-8M) and CRH (10-14 and 10-8M) was evaluated in vitro in young subjects (n = 8, age range 25-35 years) and centenarians (n = 9, age >100 years) and expressed as chemotactic index. In 9 young subjects and 6 centenarians, plasma levels of cortisol, ACTH and CRH were measured. Results: Monocyte chemotaxis towards f-MLP, ACTH(1-24) and CRH (10-8M) was well preserved in centenarians, except when the lowest concentration of CRH was used. CRH, ACTH and cortisol plasma levels were significantly higher in centenarians than in young subjects. Conclusions: The capability of monocytes from centenarians to respond to chemotactic neuropeptides is well preserved. The decreased responsiveness to the lowest concentration of CRH might be due to downregulation of CRH receptors or to defects in the intracellular signal transduction pathway. The high plasma levels of cortisol, CRH and ACTH in centenarians indicate an activation of the entire stress axis, likely counteracting the systemic inflammatory process occurring with age. This activation fits with the hypothesis that lifelong low-intensity stressors activate ancient, hormetic defense mechanisms, favoring healthy aging and longevity.

Original languageEnglish
Pages (from-to)285-289
Number of pages5
JournalNeuroImmunoModulation
Volume15
Issue number4-6
DOIs
Publication statusPublished - Nov 2008

Fingerprint

Cosyntropin
Chemotaxis
Monocytes
Adrenocorticotropic Hormone
Hydrocortisone
Corticotropin-Releasing Hormone
Defense Mechanisms
Neuropeptides
methionyl-leucyl-phenylalanine
Corticotropin-Releasing Hormone Receptors
In Vitro Techniques
Signal Transduction
Down-Regulation

Keywords

  • Centenarians
  • Inflammaging
  • Monocyte chemotaxis
  • Neuropeptides
  • Stress response

ASJC Scopus subject areas

  • Endocrinology
  • Immunology
  • Endocrine and Autonomic Systems
  • Neurology

Cite this

Genedani, S., Filaferro, M., Carone, C., Ostan, R., Bucci, L., Cevenini, E., ... Monti, D. (2008). Influence of f-MLP, ACTH(1-24) and CRH on in vitro chemotaxis of monocytes from centenarians. NeuroImmunoModulation, 15(4-6), 285-289. https://doi.org/10.1159/000156472

Influence of f-MLP, ACTH(1-24) and CRH on in vitro chemotaxis of monocytes from centenarians. / Genedani, Susanna; Filaferro, Monica; Carone, Chiara; Ostan, Rita; Bucci, Laura; Cevenini, Elisa; Franceschi, Claudio; Monti, Daniela.

In: NeuroImmunoModulation, Vol. 15, No. 4-6, 11.2008, p. 285-289.

Research output: Contribution to journalArticle

Genedani, S, Filaferro, M, Carone, C, Ostan, R, Bucci, L, Cevenini, E, Franceschi, C & Monti, D 2008, 'Influence of f-MLP, ACTH(1-24) and CRH on in vitro chemotaxis of monocytes from centenarians', NeuroImmunoModulation, vol. 15, no. 4-6, pp. 285-289. https://doi.org/10.1159/000156472
Genedani S, Filaferro M, Carone C, Ostan R, Bucci L, Cevenini E et al. Influence of f-MLP, ACTH(1-24) and CRH on in vitro chemotaxis of monocytes from centenarians. NeuroImmunoModulation. 2008 Nov;15(4-6):285-289. https://doi.org/10.1159/000156472
Genedani, Susanna ; Filaferro, Monica ; Carone, Chiara ; Ostan, Rita ; Bucci, Laura ; Cevenini, Elisa ; Franceschi, Claudio ; Monti, Daniela. / Influence of f-MLP, ACTH(1-24) and CRH on in vitro chemotaxis of monocytes from centenarians. In: NeuroImmunoModulation. 2008 ; Vol. 15, No. 4-6. pp. 285-289.
@article{c74734a89ef64043aeca3dde582bfee8,
title = "Influence of f-MLP, ACTH(1-24) and CRH on in vitro chemotaxis of monocytes from centenarians",
abstract = "Objective: The lifelong exposure to a variety of stressors activates a plethora of defense mechanisms, including the hypothalamic-pituitary-adrenal axis which releases neuropeptides affecting the immune responses. Here, we report data on the capability of monocytes from young subjects and centenarians to migrate towards chemotactic stimuli (formyl-methionyl-leucyl-phenylalanine, f-MLP; adrenocorticotropic hormone, ACTH, and corticotrophin-releasing hormone, CRH). Plasma levels of ACTH, CRH and cortisol were measured as an index of ongoing stress response. Methods: Monocyte chemotaxis towards f-MLP (10 -8M), ACTH(1-24) (10-14 and 10-8M) and CRH (10-14 and 10-8M) was evaluated in vitro in young subjects (n = 8, age range 25-35 years) and centenarians (n = 9, age >100 years) and expressed as chemotactic index. In 9 young subjects and 6 centenarians, plasma levels of cortisol, ACTH and CRH were measured. Results: Monocyte chemotaxis towards f-MLP, ACTH(1-24) and CRH (10-8M) was well preserved in centenarians, except when the lowest concentration of CRH was used. CRH, ACTH and cortisol plasma levels were significantly higher in centenarians than in young subjects. Conclusions: The capability of monocytes from centenarians to respond to chemotactic neuropeptides is well preserved. The decreased responsiveness to the lowest concentration of CRH might be due to downregulation of CRH receptors or to defects in the intracellular signal transduction pathway. The high plasma levels of cortisol, CRH and ACTH in centenarians indicate an activation of the entire stress axis, likely counteracting the systemic inflammatory process occurring with age. This activation fits with the hypothesis that lifelong low-intensity stressors activate ancient, hormetic defense mechanisms, favoring healthy aging and longevity.",
keywords = "Centenarians, Inflammaging, Monocyte chemotaxis, Neuropeptides, Stress response",
author = "Susanna Genedani and Monica Filaferro and Chiara Carone and Rita Ostan and Laura Bucci and Elisa Cevenini and Claudio Franceschi and Daniela Monti",
year = "2008",
month = "11",
doi = "10.1159/000156472",
language = "English",
volume = "15",
pages = "285--289",
journal = "NeuroImmunoModulation",
issn = "1021-7401",
publisher = "S. Karger AG",
number = "4-6",

}

TY - JOUR

T1 - Influence of f-MLP, ACTH(1-24) and CRH on in vitro chemotaxis of monocytes from centenarians

AU - Genedani, Susanna

AU - Filaferro, Monica

AU - Carone, Chiara

AU - Ostan, Rita

AU - Bucci, Laura

AU - Cevenini, Elisa

AU - Franceschi, Claudio

AU - Monti, Daniela

PY - 2008/11

Y1 - 2008/11

N2 - Objective: The lifelong exposure to a variety of stressors activates a plethora of defense mechanisms, including the hypothalamic-pituitary-adrenal axis which releases neuropeptides affecting the immune responses. Here, we report data on the capability of monocytes from young subjects and centenarians to migrate towards chemotactic stimuli (formyl-methionyl-leucyl-phenylalanine, f-MLP; adrenocorticotropic hormone, ACTH, and corticotrophin-releasing hormone, CRH). Plasma levels of ACTH, CRH and cortisol were measured as an index of ongoing stress response. Methods: Monocyte chemotaxis towards f-MLP (10 -8M), ACTH(1-24) (10-14 and 10-8M) and CRH (10-14 and 10-8M) was evaluated in vitro in young subjects (n = 8, age range 25-35 years) and centenarians (n = 9, age >100 years) and expressed as chemotactic index. In 9 young subjects and 6 centenarians, plasma levels of cortisol, ACTH and CRH were measured. Results: Monocyte chemotaxis towards f-MLP, ACTH(1-24) and CRH (10-8M) was well preserved in centenarians, except when the lowest concentration of CRH was used. CRH, ACTH and cortisol plasma levels were significantly higher in centenarians than in young subjects. Conclusions: The capability of monocytes from centenarians to respond to chemotactic neuropeptides is well preserved. The decreased responsiveness to the lowest concentration of CRH might be due to downregulation of CRH receptors or to defects in the intracellular signal transduction pathway. The high plasma levels of cortisol, CRH and ACTH in centenarians indicate an activation of the entire stress axis, likely counteracting the systemic inflammatory process occurring with age. This activation fits with the hypothesis that lifelong low-intensity stressors activate ancient, hormetic defense mechanisms, favoring healthy aging and longevity.

AB - Objective: The lifelong exposure to a variety of stressors activates a plethora of defense mechanisms, including the hypothalamic-pituitary-adrenal axis which releases neuropeptides affecting the immune responses. Here, we report data on the capability of monocytes from young subjects and centenarians to migrate towards chemotactic stimuli (formyl-methionyl-leucyl-phenylalanine, f-MLP; adrenocorticotropic hormone, ACTH, and corticotrophin-releasing hormone, CRH). Plasma levels of ACTH, CRH and cortisol were measured as an index of ongoing stress response. Methods: Monocyte chemotaxis towards f-MLP (10 -8M), ACTH(1-24) (10-14 and 10-8M) and CRH (10-14 and 10-8M) was evaluated in vitro in young subjects (n = 8, age range 25-35 years) and centenarians (n = 9, age >100 years) and expressed as chemotactic index. In 9 young subjects and 6 centenarians, plasma levels of cortisol, ACTH and CRH were measured. Results: Monocyte chemotaxis towards f-MLP, ACTH(1-24) and CRH (10-8M) was well preserved in centenarians, except when the lowest concentration of CRH was used. CRH, ACTH and cortisol plasma levels were significantly higher in centenarians than in young subjects. Conclusions: The capability of monocytes from centenarians to respond to chemotactic neuropeptides is well preserved. The decreased responsiveness to the lowest concentration of CRH might be due to downregulation of CRH receptors or to defects in the intracellular signal transduction pathway. The high plasma levels of cortisol, CRH and ACTH in centenarians indicate an activation of the entire stress axis, likely counteracting the systemic inflammatory process occurring with age. This activation fits with the hypothesis that lifelong low-intensity stressors activate ancient, hormetic defense mechanisms, favoring healthy aging and longevity.

KW - Centenarians

KW - Inflammaging

KW - Monocyte chemotaxis

KW - Neuropeptides

KW - Stress response

UR - http://www.scopus.com/inward/record.url?scp=57149112134&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57149112134&partnerID=8YFLogxK

U2 - 10.1159/000156472

DO - 10.1159/000156472

M3 - Article

C2 - 19047806

AN - SCOPUS:57149112134

VL - 15

SP - 285

EP - 289

JO - NeuroImmunoModulation

JF - NeuroImmunoModulation

SN - 1021-7401

IS - 4-6

ER -