Influence of growth hormone-releasing hormone (GHRH) on phytoemoagglutinin-induced lymphocyte activation: Comparison of two synthetic forms. GHRH and PHA-induced lymphocyte activation

A. Valtorta, A. Moretta, R. Maccario, M. Bozzola, F. Severi

Research output: Contribution to journalArticlepeer-review

Abstract

The in vitro effect of synthetic human growth hormone-releasing hormone (GHRH) on mitogen-induced lymphocyte proliferation and lymphokine sercretion was investigated. Peripheral blood mononuclear cells (PBMC) of healthy adults were incubated in the presence and absence of increasing concentrations (from 0.006 to 50 μg/ml) of two forms of GHRH differing in amino-acid sequence (GHRH 1-44 and GHRH 1-29) or of increasing concentrations (from 0.0012 to 20 U/ml) of recombinant human insulin (rh-insulin). Low concentrations of GHRH 1-29 increased phytoemoagglutinin (PHA)-induced lymphoproliferation, while high concentrations inhibited lymphocyte response, interleukin-2 (IL-2) secretion and IL-2 receptor expression on activated cells. A toxic effect was excluded since no differences in cell viability were observed between cells cultured with and without hormone. GHRH 1-44 did not affect PHA-induced lymphoproliferation, IL-2 production and IL-2 receptor expression. Low concentrations of rh-insulin increased PHA-elicited lymphoproliferation, while high concentrations did not decrease lymphocyte response. The present study suggests that GHRH modulates in vitro human T lymphocyte functions.

Original languageEnglish
Pages (from-to)51-59
Number of pages9
JournalThymus
Volume18
Issue number1
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Immunology

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