TY - JOUR
T1 - Influence of lignans depletion on murine mammary gland morphology
AU - Rossini, Anna
AU - Zanobbio, Laura
AU - Palazzo, Marco
AU - Sfondrini, Lucia
AU - Morelli, Daniele
AU - Tagliabue, Elda
AU - Balsari, Andrea
AU - Rumio, Cristiano
PY - 2010/2
Y1 - 2010/2
N2 - Different studies have focused on the effects of phytoestrogens- supplemented diets on mammary gland morphogenesis and breast cancer risk; however, particular dieting behaviors and food choices may result in a reduction of the natural source of phytoestrogens. The evaluation of a reduced phytoestrogens intake effect by depletion without modifying other dietary ingredients is hard. Since lignans, the largest contributors to phytoestrogens intake in Western diets, are metabolized into bioactive compounds by gut bacteria, long-term antibiotic treatments, inducing intestinal microflora disruption, may reduce enterolactone availability. To elucidate the effect of phytoestrogens lack on mammary tissue morphogenesis, female FVB mice were treated with gentamicin or metronidazole/ciprofloxacin from the age of 6 to 7 wk. After 21 wk, enterolactone urine levels were 120.07 ± 20.5 ng/ml in untreated mice, 30.4 ± 24.46 ng/ml in metronidazole/ciprofloxacin-treated mice, and 3.29 ± 4.38 ng/ml in gentamicin-treated mice. Histological analysis revealed no significant alterations of mammary morphology in metronidazole/ciprofloxacin-treated mice, whereas gentamicin-treated mice showed increase of ducts number and duct-tree branching vs. controls. These findings indicate that normal mammary tissue size and shape are maintained even in the presence of low levels of lignans and suggest that only a complete depletion of these compounds induced significant alterations of mammary gland structure.
AB - Different studies have focused on the effects of phytoestrogens- supplemented diets on mammary gland morphogenesis and breast cancer risk; however, particular dieting behaviors and food choices may result in a reduction of the natural source of phytoestrogens. The evaluation of a reduced phytoestrogens intake effect by depletion without modifying other dietary ingredients is hard. Since lignans, the largest contributors to phytoestrogens intake in Western diets, are metabolized into bioactive compounds by gut bacteria, long-term antibiotic treatments, inducing intestinal microflora disruption, may reduce enterolactone availability. To elucidate the effect of phytoestrogens lack on mammary tissue morphogenesis, female FVB mice were treated with gentamicin or metronidazole/ciprofloxacin from the age of 6 to 7 wk. After 21 wk, enterolactone urine levels were 120.07 ± 20.5 ng/ml in untreated mice, 30.4 ± 24.46 ng/ml in metronidazole/ciprofloxacin-treated mice, and 3.29 ± 4.38 ng/ml in gentamicin-treated mice. Histological analysis revealed no significant alterations of mammary morphology in metronidazole/ciprofloxacin-treated mice, whereas gentamicin-treated mice showed increase of ducts number and duct-tree branching vs. controls. These findings indicate that normal mammary tissue size and shape are maintained even in the presence of low levels of lignans and suggest that only a complete depletion of these compounds induced significant alterations of mammary gland structure.
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U2 - 10.1080/01635580903305284
DO - 10.1080/01635580903305284
M3 - Article
C2 - 20099198
AN - SCOPUS:76749132226
VL - 62
SP - 237
EP - 242
JO - Nutrition and Cancer
JF - Nutrition and Cancer
SN - 0163-5581
IS - 2
ER -