Influence of local environment on the differentiation of neural stem cells engrafted onto the injured spinal cord

Lucia Ricci-Vitiani, Patrizia Casalbore, Giovanna Petrucci, Liverana Lauretti, Nicola Montano, Luigi Maria Larocca, Maria Laura Falchetti, Dario Giuseppe Lombardi, Valeria Di Giorgio Gerevini, Carlo Cenciarelli, Quintino Giorgio D'Alessandris, Eduardo Fernandez, Ruggero De Maria, Giulio Maira, Cesare Peschle, Eugenio Parati, Roberto Pallini

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Objectives: In vitro, neural stem cells (NSCs) proliferate as undifferentiated spheroids and differentiate into neurons, astrocytes and oligodendrocytes. These features make NSCs suitable for spinal cord (SC) reconstruction. However, in vivo experiments have demonstrated that in the injured SC transplanted NSCs either remain undifferentiated or differentiate into the astrocytic phenotype. The microenvironment of the injured SC is believed to play a crucial role in driving the differentiation of the engrafted NSCs. Here, we tested the hypothesis that inflammatory cytokines (ICs) may be involved in the restricted differentiation of NSCs after grafting onto the injured SC. Methods: As the first step, we used immunohistochemistry to analyse the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and interferon (IFN)-γ in the normal SC of mice and following traumatic injury. Then, we investigated whether a combination of TNF-α, IL-1β and IFN-γ may affect the phenotype of murine NSCs in vitro. Results: We found that TNF-α, IL- 1β and IFN-γ, which are absent in the normal SC, are all expressed in the injured SC and the expression of these cytokines follows a timely tuned fashion with IFN-γ being detectable as long as 4 weeks after injury. In culture, exposure of proliferating NSCs to a combination of TNF-α, IL-1β and IFN-γ was per se sufficient to induce the astrocytic differentiation of these cells even in the absence of serum. Conclusions: In the traumatically injured SC, differentiation of engrafted NSCs is restricted towards the astrocytic lineage because of the inflammatory environment. ICs are likely to play a major role in differentiation of NSCs in the in vivo conditions.

Original languageEnglish
Pages (from-to)488-492
Number of pages5
JournalNeurological Research
Volume28
Issue number5
DOIs
Publication statusPublished - Jul 2006

Fingerprint

Neural Stem Cells
Spinal Cord
Interferons
Interleukin-1
Tumor Necrosis Factor-alpha
Cytokines
Phenotype
Oligodendroglia
Wounds and Injuries
Astrocytes
Cell Differentiation
Immunohistochemistry
Neurons

Keywords

  • Astrocytic differentiation
  • Inflammatory cytokines
  • Neural stem cells
  • Spinal cord injury

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Ricci-Vitiani, L., Casalbore, P., Petrucci, G., Lauretti, L., Montano, N., Larocca, L. M., ... Pallini, R. (2006). Influence of local environment on the differentiation of neural stem cells engrafted onto the injured spinal cord. Neurological Research, 28(5), 488-492. https://doi.org/10.1179/016164106X115134

Influence of local environment on the differentiation of neural stem cells engrafted onto the injured spinal cord. / Ricci-Vitiani, Lucia; Casalbore, Patrizia; Petrucci, Giovanna; Lauretti, Liverana; Montano, Nicola; Larocca, Luigi Maria; Falchetti, Maria Laura; Lombardi, Dario Giuseppe; Gerevini, Valeria Di Giorgio; Cenciarelli, Carlo; D'Alessandris, Quintino Giorgio; Fernandez, Eduardo; De Maria, Ruggero; Maira, Giulio; Peschle, Cesare; Parati, Eugenio; Pallini, Roberto.

In: Neurological Research, Vol. 28, No. 5, 07.2006, p. 488-492.

Research output: Contribution to journalArticle

Ricci-Vitiani, L, Casalbore, P, Petrucci, G, Lauretti, L, Montano, N, Larocca, LM, Falchetti, ML, Lombardi, DG, Gerevini, VDG, Cenciarelli, C, D'Alessandris, QG, Fernandez, E, De Maria, R, Maira, G, Peschle, C, Parati, E & Pallini, R 2006, 'Influence of local environment on the differentiation of neural stem cells engrafted onto the injured spinal cord', Neurological Research, vol. 28, no. 5, pp. 488-492. https://doi.org/10.1179/016164106X115134
Ricci-Vitiani, Lucia ; Casalbore, Patrizia ; Petrucci, Giovanna ; Lauretti, Liverana ; Montano, Nicola ; Larocca, Luigi Maria ; Falchetti, Maria Laura ; Lombardi, Dario Giuseppe ; Gerevini, Valeria Di Giorgio ; Cenciarelli, Carlo ; D'Alessandris, Quintino Giorgio ; Fernandez, Eduardo ; De Maria, Ruggero ; Maira, Giulio ; Peschle, Cesare ; Parati, Eugenio ; Pallini, Roberto. / Influence of local environment on the differentiation of neural stem cells engrafted onto the injured spinal cord. In: Neurological Research. 2006 ; Vol. 28, No. 5. pp. 488-492.
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AU - Ricci-Vitiani, Lucia

AU - Casalbore, Patrizia

AU - Petrucci, Giovanna

AU - Lauretti, Liverana

AU - Montano, Nicola

AU - Larocca, Luigi Maria

AU - Falchetti, Maria Laura

AU - Lombardi, Dario Giuseppe

AU - Gerevini, Valeria Di Giorgio

AU - Cenciarelli, Carlo

AU - D'Alessandris, Quintino Giorgio

AU - Fernandez, Eduardo

AU - De Maria, Ruggero

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AU - Parati, Eugenio

AU - Pallini, Roberto

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KW - Neural stem cells

KW - Spinal cord injury

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