Influence of novel nano-titanium implant surface on human osteoblast behavior and growth

Stefano Tetè, Filiberto Mastrangelo, Raimondo Quaresima, Raffaele Vinci, Gilberto Sammartino, Liborio Stuppia, Enrico Gherlone

Research output: Contribution to journalArticlepeer-review


Purpose: The aim of the study is to investigate human osteoblast-like cell behavior and growth in the presence of 3 different titanium implant surfaces. Materials: Human stem cells were first obtained and then sorted by fluorescence-activated cell sorter from mesenchymal stem cell clusters of human dental papilla. The obtained human dental papilla stem cells were induced to differentiate into osteoblast-like cells and were then analyzed by reverse transcriptase polymerase chain reaction and Western blot analyses. The cells proliferated and were cultured onto 3 different titanium discs (sandblasted, sandblasted and large-grit acid-etched, and full contact coverage [FCC]) and analyzed by scanning electron microscope. Results: In all analyses samples, a high cell activity was observed, with typical osteoblast mature morphostructural response on rough surface. The high number of osteoblast-like cells was found on titanium FCC discs. At the same time, scanning electron microscope analysis confirmed the high biocompatibility of this surface. Conclusion: The rapid maturation of the osteoblast-like cells on FCC titanium surface suggests that this structure could play a central role during initial phases of bone healing processes.

Original languageEnglish
Pages (from-to)520-531
Number of pages12
JournalImplant Dentistry
Issue number6
Publication statusPublished - Dec 2010


  • full contact coverage surface
  • human dental papilla stem cells
  • osteogenic differentiation
  • titanium surface

ASJC Scopus subject areas

  • Oral Surgery


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