Influence of Pertussis toxin on CD1a isoform expression in human dendritic cells

Angelo Martino, Elisabetta Volpe, Giovanni Auricchio, Vittorio Colizzi, Patrizia Morena Baldini

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Pertussis toxin (PTX) is an exotoxin produced by Bordetella pertussis. It is known to exert adjuvant activities inducing Th1-launched immune responses. In this study, we show that PTX can selectively block the expression of CD1a isoform during the differentiation of human monocytes into dendritic cells. In fact, dendritic cells differentiated from monocytes in the presence of PTX do not express CD1a on their surface, unlike CD1b and CD1c isoforms, which are normally regulated. The impaired CD1a expression on cell membrane depends, at least partially, on decreased mRNA transcription and does not affect cellular capability to respond to other maturation stimuli. Since CD1a+ dendritic cells are involved in the early steps of primary immune response, the interference of PTX in the CD1a expression may be relevant for its employment as adjuvant.

Original languageEnglish
Pages (from-to)153-159
Number of pages7
JournalJournal of Clinical Immunology
Volume26
Issue number2
DOIs
Publication statusPublished - Mar 2006

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Pertussis Toxin
Dendritic Cells
Protein Isoforms
Monocytes
Exotoxins
Bordetella pertussis
Cell Membrane
Messenger RNA

Keywords

  • CD1
  • dendritic cells
  • differentiation
  • Pertussis toxin

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Influence of Pertussis toxin on CD1a isoform expression in human dendritic cells. / Martino, Angelo; Volpe, Elisabetta; Auricchio, Giovanni; Colizzi, Vittorio; Baldini, Patrizia Morena.

In: Journal of Clinical Immunology, Vol. 26, No. 2, 03.2006, p. 153-159.

Research output: Contribution to journalArticle

Martino, Angelo ; Volpe, Elisabetta ; Auricchio, Giovanni ; Colizzi, Vittorio ; Baldini, Patrizia Morena. / Influence of Pertussis toxin on CD1a isoform expression in human dendritic cells. In: Journal of Clinical Immunology. 2006 ; Vol. 26, No. 2. pp. 153-159.
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