Influence of RNA synthesis on DNA-repair replication in human cell extracts

Guido Frosina; Richard D. Wood

doi:10.1016/0165-7992(90)90074-T

Influence of RNA synthesis on DNA-repair replication in human cell extracts

Guido Frosina, Richard D. Wood

A.O.U. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA)

Research output: Contribution to journal › Article › peer-review

Abstract

An in vitro system allowing human cell extracts to carry out excision repair in ultraviolet light-irradiated, closed circular DNA was used to study the influence of transcription on repair synthesis. RNA synthesis from plasmid DNA containing bacterial transcription units was obtained by addition of Escherichia coli RNA polymerase and ribonucleoside triphosphates to the repair-incubation mixture. No increase in UV-stimulated repair replication was observed under transcriptional conditions; in fact, an inhibition of repair replication occurred, possibly due to impairment of DNA polymerization. This is unlike the in vivo situation where preferential DNA repair of transcribed genes has been found after UV-irradiation. Possible factors important for preferential repair of expressed genes in mammalian cells are discussed.

Original language	English
Pages (from-to)	287-293
Number of pages	7
Journal	Mutation Research Letters
Volume	244
Issue number	4
DOIs	https://doi.org/10.1016/0165-7992(90)90074-T
Publication status	Published - 1990

Keywords

DNA repair replication
Excision repair
RNA polymerase
RNA synthesis
Transcription

ASJC Scopus subject areas

Genetics
Toxicology
Medicine(all)

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10.1016/0165-7992(90)90074-T

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title = "Influence of RNA synthesis on DNA-repair replication in human cell extracts",

abstract = "An in vitro system allowing human cell extracts to carry out excision repair in ultraviolet light-irradiated, closed circular DNA was used to study the influence of transcription on repair synthesis. RNA synthesis from plasmid DNA containing bacterial transcription units was obtained by addition of Escherichia coli RNA polymerase and ribonucleoside triphosphates to the repair-incubation mixture. No increase in UV-stimulated repair replication was observed under transcriptional conditions; in fact, an inhibition of repair replication occurred, possibly due to impairment of DNA polymerization. This is unlike the in vivo situation where preferential DNA repair of transcribed genes has been found after UV-irradiation. Possible factors important for preferential repair of expressed genes in mammalian cells are discussed.",

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AU - Frosina, Guido

AU - Wood, Richard D.

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AB - An in vitro system allowing human cell extracts to carry out excision repair in ultraviolet light-irradiated, closed circular DNA was used to study the influence of transcription on repair synthesis. RNA synthesis from plasmid DNA containing bacterial transcription units was obtained by addition of Escherichia coli RNA polymerase and ribonucleoside triphosphates to the repair-incubation mixture. No increase in UV-stimulated repair replication was observed under transcriptional conditions; in fact, an inhibition of repair replication occurred, possibly due to impairment of DNA polymerization. This is unlike the in vivo situation where preferential DNA repair of transcribed genes has been found after UV-irradiation. Possible factors important for preferential repair of expressed genes in mammalian cells are discussed.

KW - DNA repair replication

KW - Excision repair

KW - RNA polymerase

KW - RNA synthesis

KW - Transcription

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Influence of RNA synthesis on DNA-repair replication in human cell extracts

Abstract

Keywords

ASJC Scopus subject areas

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