Abstract
An in vitro system allowing human cell extracts to carry out excision repair in ultraviolet light-irradiated, closed circular DNA was used to study the influence of transcription on repair synthesis. RNA synthesis from plasmid DNA containing bacterial transcription units was obtained by addition of Escherichia coli RNA polymerase and ribonucleoside triphosphates to the repair-incubation mixture. No increase in UV-stimulated repair replication was observed under transcriptional conditions; in fact, an inhibition of repair replication occurred, possibly due to impairment of DNA polymerization. This is unlike the in vivo situation where preferential DNA repair of transcribed genes has been found after UV-irradiation. Possible factors important for preferential repair of expressed genes in mammalian cells are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 287-293 |
| Number of pages | 7 |
| Journal | Mutation Research Letters |
| Volume | 244 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1990 |
Keywords
- DNA repair replication
- Excision repair
- RNA polymerase
- RNA synthesis
- Transcription
ASJC Scopus subject areas
- Genetics
- Toxicology
- Medicine(all)