Influence of sampling window size and orientation on parafoveal cone packing density

Marco Lombardo, Sebastiano Serrao, Pietro Ducoli, Giuseppe Lombardo

Research output: Contribution to journalArticlepeer-review


We assessed the agreement between sampling windows of different size and orientation on packing density estimates in images of the parafoveal cone mosaic acquired using a flood-illumination adaptive optics retinal camera. Horizontal and vertical oriented sampling windows of different size (320x160 μm, 160x80 μm and 80x40 μm) were selected in two retinal locations along the horizontal meridian in one eye of ten subjects. At each location, cone density tended to decline with decreasing sampling area. Although the differences in cone density estimates were not statistically significant, Bland-Altman plots showed that the agreement between cone density estimated within the different sampling window conditions was moderate. The percentage of the preferred packing arrangements of cones by Voronoi tiles was slightly affected by window size and orientation. The results illustrated the high importance of specifying the size and orientation of the sampling window used to derive cone metric estimates to facilitate comparison of different studies.

Original languageEnglish
Pages (from-to)1318-1331
Number of pages14
JournalBiomedical Optics Express
Issue number8
Publication statusPublished - 2013

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology


Dive into the research topics of 'Influence of sampling window size and orientation on parafoveal cone packing density'. Together they form a unique fingerprint.

Cite this