Influence of some detoxification enzyme polymorphisms on cytogenetic biomarkers between individuals exposed to very low doses of 1,3-butadiene

Nenad Bukvic, Piero Lovreglio, Margherita Fanelli, Francesco C. Susca, Andrea Ballini, Patrizia Lastella, Vito Foà, Silvia Fustinoni, Leonardo Soleo, Ginevra Guanti

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVE: To evaluate the variation of some biomarkers related to the level of enzymatic activity dependent on the different polymorphisms. METHODS: We studied 27 butadiene-exposed workers and 37 controls using different biomarkers of the genotoxic effect. The genotypes were determined using polymerase chain reaction and restriction fragment length polymorphism- polymerase chain reaction techniques; the subjects were assigned to a specific group based on the microsomal epoxide hydrolase (mEH) activity predicted by their genotype (low, intermediate, high). RESULTS: The studied biomarkers were not able to discriminate between exposed and control individuals, but sister chromatid exchange (SCE) and high frequency cells were influenced by smoking habits. Smokers having fast microsomal epoxide hydrolase activity showed higher SCE frequency (7.61) respect to those presenting intermediate (5.86) or slow (6.65) enzymatic activity. CONCLUSIONS: On the basis of these results, can we suppose the existence of an "intermediate genotype" advantage (at least for induction of SCE)?

Original languageEnglish
Pages (from-to)811-821
Number of pages11
JournalJournal of Occupational and Environmental Medicine
Volume51
Issue number7
DOIs
Publication statusPublished - Jul 2009

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

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