Abstract
In the present study the ability of subinhibitory concentrations (sub-MICs) of brodimoprim (a new 2,4-dimethoxybenzylpyri-midine) to interfere with some important aspects of bacterial cell function, such as surface hydrophobicity, fimbriation, motility and adhesiveness to mucosal cells, was investigated in comparison with those of trimethoprim. The inhibitory behavior of both diaminopyrimidines concerning hydrophobicity and hemagglutination (fimbriation) were essentially the same, while for adhesiveness and motility brodimoprim was more effective than trimethoprim. Diaminopyrimidines have high affinity for the bacterial enzyme dihydrofolate reductase, and this reduces the synthesis of essential purines and as a conse-quence of DNA and proteins. Our findings indicate that the synthesis and/or the expression of surface adhesins, which are proteins, was also affected by both brodimoprim and trimethoprim, the former being more active.
| Original language | English |
|---|---|
| Pages (from-to) | 50-58 |
| Number of pages | 9 |
| Journal | Chemotherapy |
| Volume | 41 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1995 |
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Keywords
- Adhesiveness
- Brodimoprim
- Escherichia coli
- Fimbriation
- Hydrophobicity
- Motility
- Subinhibitory concentration
- Trimethoprim
ASJC Scopus subject areas
- Infectious Diseases
- Oncology
- Pharmacology (medical)
- Drug Discovery
- Pharmacology
Cite this
Influence of subinhibitory concentrations of brodimoprim and trimethoprim on the adhesiveness, hydrophobicity, hemagglutination and motility of escherichia coli. / Braga, P. C.; Sasso, M. Dal; Maci, S.; Reggio, S.; Piatti, G.
In: Chemotherapy, Vol. 41, No. 1, 1995, p. 50-58.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Influence of subinhibitory concentrations of brodimoprim and trimethoprim on the adhesiveness, hydrophobicity, hemagglutination and motility of escherichia coli
AU - Braga, P. C.
AU - Sasso, M. Dal
AU - Maci, S.
AU - Reggio, S.
AU - Piatti, G.
PY - 1995
Y1 - 1995
N2 - In the present study the ability of subinhibitory concentrations (sub-MICs) of brodimoprim (a new 2,4-dimethoxybenzylpyri-midine) to interfere with some important aspects of bacterial cell function, such as surface hydrophobicity, fimbriation, motility and adhesiveness to mucosal cells, was investigated in comparison with those of trimethoprim. The inhibitory behavior of both diaminopyrimidines concerning hydrophobicity and hemagglutination (fimbriation) were essentially the same, while for adhesiveness and motility brodimoprim was more effective than trimethoprim. Diaminopyrimidines have high affinity for the bacterial enzyme dihydrofolate reductase, and this reduces the synthesis of essential purines and as a conse-quence of DNA and proteins. Our findings indicate that the synthesis and/or the expression of surface adhesins, which are proteins, was also affected by both brodimoprim and trimethoprim, the former being more active.
AB - In the present study the ability of subinhibitory concentrations (sub-MICs) of brodimoprim (a new 2,4-dimethoxybenzylpyri-midine) to interfere with some important aspects of bacterial cell function, such as surface hydrophobicity, fimbriation, motility and adhesiveness to mucosal cells, was investigated in comparison with those of trimethoprim. The inhibitory behavior of both diaminopyrimidines concerning hydrophobicity and hemagglutination (fimbriation) were essentially the same, while for adhesiveness and motility brodimoprim was more effective than trimethoprim. Diaminopyrimidines have high affinity for the bacterial enzyme dihydrofolate reductase, and this reduces the synthesis of essential purines and as a conse-quence of DNA and proteins. Our findings indicate that the synthesis and/or the expression of surface adhesins, which are proteins, was also affected by both brodimoprim and trimethoprim, the former being more active.
KW - Adhesiveness
KW - Brodimoprim
KW - Escherichia coli
KW - Fimbriation
KW - Hydrophobicity
KW - Motility
KW - Subinhibitory concentration
KW - Trimethoprim
UR - http://www.scopus.com/inward/record.url?scp=0028942031&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028942031&partnerID=8YFLogxK
U2 - 10.1159/000239324
DO - 10.1159/000239324
M3 - Article
C2 - 7875023
AN - SCOPUS:0028942031
VL - 41
SP - 50
EP - 58
JO - Chemotherapy
JF - Chemotherapy
SN - 0009-3157
IS - 1
ER -