Influence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes

Prabhani U. Atukorale, Yu Sang Yang, Ahmet Bekdemir, Randy P. Carney, Paulo J. Silva, Nicki Watson, Francesco Stellacci, Darrell J. Irvine

Research output: Contribution to journalArticle

Abstract

Erythrocytes are attractive as potential cell-based drug carriers because of their abundance and long lifespan in vivo. Existing methods for loading drug cargos into erythrocytes include hypotonic treatments, electroporation, and covalent attachment onto the membrane, all of which require ex vivo manipulation. Here, we characterized the properties of amphiphilic gold nanoparticles (amph-AuNPs), comprised of a ∼2.3 nm gold core and an amphiphilic ligand shell, which are able to embed spontaneously within erythrocyte membranes and might provide a means to load drugs into red blood cells (RBCs) directly in vivo. Particle interaction with RBC membranes occurred rapidly at physiological temperature. We further show that amph-AuNP uptake by RBCs was limited by the glycocalyx and was particularly influenced by sialic acids on cell surface proteoglycans. Using a reductionist model membrane system with synthetic lipid vesicles, we confirmed the importance of membrane fluidity and the glycocalyx in regulating amph-AuNP/membrane interactions. These results thus provide evidence for the interaction of amph-AuNPs with erythrocyte membranes and identify key membrane components that govern this interaction, providing a framework for the development of amph-AuNP-carrying erythrocyte 'pharmacytes' in vivo.

Original languageEnglish
Pages (from-to)11420-11432
Number of pages13
JournalNanoscale
Volume7
Issue number26
DOIs
Publication statusPublished - Jul 14 2015

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Cell membranes
Gold
Association reactions
Nanoparticles
Membranes
Chemical analysis
Blood
Sialic Acids
Drug Carriers
Particle interactions
Fluidity
Proteoglycans
Pharmaceutical Preparations
Lipids
Ligands
Cells
Acids

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Atukorale, P. U., Yang, Y. S., Bekdemir, A., Carney, R. P., Silva, P. J., Watson, N., ... Irvine, D. J. (2015). Influence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes. Nanoscale, 7(26), 11420-11432. https://doi.org/10.1039/c5nr01355k

Influence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes. / Atukorale, Prabhani U.; Yang, Yu Sang; Bekdemir, Ahmet; Carney, Randy P.; Silva, Paulo J.; Watson, Nicki; Stellacci, Francesco; Irvine, Darrell J.

In: Nanoscale, Vol. 7, No. 26, 14.07.2015, p. 11420-11432.

Research output: Contribution to journalArticle

Atukorale, PU, Yang, YS, Bekdemir, A, Carney, RP, Silva, PJ, Watson, N, Stellacci, F & Irvine, DJ 2015, 'Influence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes', Nanoscale, vol. 7, no. 26, pp. 11420-11432. https://doi.org/10.1039/c5nr01355k
Atukorale, Prabhani U. ; Yang, Yu Sang ; Bekdemir, Ahmet ; Carney, Randy P. ; Silva, Paulo J. ; Watson, Nicki ; Stellacci, Francesco ; Irvine, Darrell J. / Influence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes. In: Nanoscale. 2015 ; Vol. 7, No. 26. pp. 11420-11432.
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