Influence of tumor on adriamycin concentration in blood cells

Massimo Broggini, Tina Colombo, Silvio Garattini, Maria Grazia Donelli

Research output: Contribution to journalArticle

Abstract

The relative distribution of adriamycin to plasma and blood cells after IV injection of 10 mg/kg was investigated in CD rats bearing intramuscular 256 Walker carcinosarcomas 15 days old. The drug was measured by a fluorimetric procedure and the amount of unchanged compound was separated from metabolites and quantitated by means of a TLC scanning fluorescence technique. In the presence of a tumor much lower hematocrit values are found, with marked anemia and thrombocytopenia associated with leukocytosis. These modified hematologic parameters account for an altered pattern of drug distribution. The low number of blood cells per milliliter results in a smaller amount of drug being present in the cellular fraction, so that more of the compound (even twice as much) is made available in plasma. Changes in adriamycin concentrations per unit volume or cell of each cell type are inversely related to changes in their relative number per milliliter. The only cell fraction where the drug increase per cell or cubic micrometer does not compensate the marked reduction in cell count observed in the presence of tumor is the platelet fraction, in which adriamycin amounts are 25% or less of those observed in the blood of normal rats, indicating that these blood cells become saturated in tumor-bearing animals.

Original languageEnglish
Pages (from-to)209-212
Number of pages4
JournalCancer Chemotherapy and Pharmacology
Volume4
Issue number3
DOIs
Publication statusPublished - 1980

Fingerprint

Doxorubicin
Tumors
Blood Cells
Blood
Bearings (structural)
Cells
Pharmaceutical Preparations
Rats
Neoplasms
Walker Carcinoma 256
Plasmas
Blood Cell Count
Leukocytosis
Metabolites
Platelets
Plasma Cells
Cell Size
Hematocrit
Thrombocytopenia
Anemia

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pharmacology

Cite this

Influence of tumor on adriamycin concentration in blood cells. / Broggini, Massimo; Colombo, Tina; Garattini, Silvio; Donelli, Maria Grazia.

In: Cancer Chemotherapy and Pharmacology, Vol. 4, No. 3, 1980, p. 209-212.

Research output: Contribution to journalArticle

@article{86c5ef6dbc5b47f99989ad3f4ac4dff0,
title = "Influence of tumor on adriamycin concentration in blood cells",
abstract = "The relative distribution of adriamycin to plasma and blood cells after IV injection of 10 mg/kg was investigated in CD rats bearing intramuscular 256 Walker carcinosarcomas 15 days old. The drug was measured by a fluorimetric procedure and the amount of unchanged compound was separated from metabolites and quantitated by means of a TLC scanning fluorescence technique. In the presence of a tumor much lower hematocrit values are found, with marked anemia and thrombocytopenia associated with leukocytosis. These modified hematologic parameters account for an altered pattern of drug distribution. The low number of blood cells per milliliter results in a smaller amount of drug being present in the cellular fraction, so that more of the compound (even twice as much) is made available in plasma. Changes in adriamycin concentrations per unit volume or cell of each cell type are inversely related to changes in their relative number per milliliter. The only cell fraction where the drug increase per cell or cubic micrometer does not compensate the marked reduction in cell count observed in the presence of tumor is the platelet fraction, in which adriamycin amounts are 25{\%} or less of those observed in the blood of normal rats, indicating that these blood cells become saturated in tumor-bearing animals.",
author = "Massimo Broggini and Tina Colombo and Silvio Garattini and Donelli, {Maria Grazia}",
year = "1980",
doi = "10.1007/BF00254021",
language = "English",
volume = "4",
pages = "209--212",
journal = "Cancer Chemotherapy and Pharmacology",
issn = "0344-5704",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Influence of tumor on adriamycin concentration in blood cells

AU - Broggini, Massimo

AU - Colombo, Tina

AU - Garattini, Silvio

AU - Donelli, Maria Grazia

PY - 1980

Y1 - 1980

N2 - The relative distribution of adriamycin to plasma and blood cells after IV injection of 10 mg/kg was investigated in CD rats bearing intramuscular 256 Walker carcinosarcomas 15 days old. The drug was measured by a fluorimetric procedure and the amount of unchanged compound was separated from metabolites and quantitated by means of a TLC scanning fluorescence technique. In the presence of a tumor much lower hematocrit values are found, with marked anemia and thrombocytopenia associated with leukocytosis. These modified hematologic parameters account for an altered pattern of drug distribution. The low number of blood cells per milliliter results in a smaller amount of drug being present in the cellular fraction, so that more of the compound (even twice as much) is made available in plasma. Changes in adriamycin concentrations per unit volume or cell of each cell type are inversely related to changes in their relative number per milliliter. The only cell fraction where the drug increase per cell or cubic micrometer does not compensate the marked reduction in cell count observed in the presence of tumor is the platelet fraction, in which adriamycin amounts are 25% or less of those observed in the blood of normal rats, indicating that these blood cells become saturated in tumor-bearing animals.

AB - The relative distribution of adriamycin to plasma and blood cells after IV injection of 10 mg/kg was investigated in CD rats bearing intramuscular 256 Walker carcinosarcomas 15 days old. The drug was measured by a fluorimetric procedure and the amount of unchanged compound was separated from metabolites and quantitated by means of a TLC scanning fluorescence technique. In the presence of a tumor much lower hematocrit values are found, with marked anemia and thrombocytopenia associated with leukocytosis. These modified hematologic parameters account for an altered pattern of drug distribution. The low number of blood cells per milliliter results in a smaller amount of drug being present in the cellular fraction, so that more of the compound (even twice as much) is made available in plasma. Changes in adriamycin concentrations per unit volume or cell of each cell type are inversely related to changes in their relative number per milliliter. The only cell fraction where the drug increase per cell or cubic micrometer does not compensate the marked reduction in cell count observed in the presence of tumor is the platelet fraction, in which adriamycin amounts are 25% or less of those observed in the blood of normal rats, indicating that these blood cells become saturated in tumor-bearing animals.

UR - http://www.scopus.com/inward/record.url?scp=0018852730&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018852730&partnerID=8YFLogxK

U2 - 10.1007/BF00254021

DO - 10.1007/BF00254021

M3 - Article

C2 - 7397946

AN - SCOPUS:0018852730

VL - 4

SP - 209

EP - 212

JO - Cancer Chemotherapy and Pharmacology

JF - Cancer Chemotherapy and Pharmacology

SN - 0344-5704

IS - 3

ER -