Influence of vasostatins, the chromogranin A-derived peptides, on the working heart of the eel (Anguilla anguilla): Negative inotropy and mechanism of action

Sandra Imbrogno, Tommaso Angelone, Angelo Corti, Cristina Adamo, Karen B. Helle, Bruno Tota

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

We have studied the effects of exogenous human recombinant Vasostatin-1 (VS-1), Vasostatin-2 (VS-2) and the human Chromogranin A (CGA) 7-57 synthetic peptides on the mechanical performance of the isolated and perfused working eel (Anguilla anguilla) heart. Under basal conditions, the three peptides decreased stroke volume (SV) and stroke work (SW), thus exerting negative inotropism. The VS-1-mediated negative inotropism was abolished by exposure to inhibitors of either Gi/o protein (pertussis toxin; PTx) or M1 muscarinic receptors (Pirenzepine) or calcium (Lantanum and Diltiazem) and potassium (Ba 2+, 4-aminopyridine, tetraethylammonium, glibenclamide) channels, while it required an intact endocardial endothelium (EE). Using N G-monomethyl-l-arginine (l-NMMA) as an inhibitor of nitric oxide (NO) synthase (NOS), and hemoglobin as a NO scavenger, we demonstrated the obligatory role of NO signaling in mediating the vasostatin response. Pretreatment with either a specific inhibitor of soluble guanylate cyclase (GC) 1H-(1,2,4)oxadiazolo-(4,3-a)quinoxalin-1-one (ODQ), or the inhibitor of the cGMP-activated protein kinase (PKG) KT 5823, abolished the VS-1-mediated inotropism, indicating the cGMP-PKG component as a crucial target of NO signaling. Of note, VS-1 was effective in counteracting the adrenergic (Isoproterenol and Phenylephrine)-mediated positive inotropism. These findings provide the first evidence that vasostatins exert cardiotropic action in fish, thus suggesting their long evolutionary history as well as their species-specific mechanisms of action.

Original languageEnglish
Pages (from-to)20-28
Number of pages9
JournalGeneral and Comparative Endocrinology
Volume139
Issue number1
DOIs
Publication statusPublished - Oct 2004

Fingerprint

Anguilla
Chromogranin A
Eels
Anguilla anguilla
Muscle Contraction
eel
nitric oxide
mechanism of action
heart
peptides
stroke
Nitric Oxide
Peptides
4-aminopyridine
quinoxalines
glibenclamide
pertussis toxin
guanylate cyclase
phenylephrine
synthetic peptides

Keywords

  • Calcium channels
  • Catecholamines
  • CGA-derived peptides
  • Eel heart
  • Nitric oxide
  • Potassium channels

ASJC Scopus subject areas

  • Endocrinology

Cite this

Influence of vasostatins, the chromogranin A-derived peptides, on the working heart of the eel (Anguilla anguilla) : Negative inotropy and mechanism of action. / Imbrogno, Sandra; Angelone, Tommaso; Corti, Angelo; Adamo, Cristina; Helle, Karen B.; Tota, Bruno.

In: General and Comparative Endocrinology, Vol. 139, No. 1, 10.2004, p. 20-28.

Research output: Contribution to journalArticle

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abstract = "We have studied the effects of exogenous human recombinant Vasostatin-1 (VS-1), Vasostatin-2 (VS-2) and the human Chromogranin A (CGA) 7-57 synthetic peptides on the mechanical performance of the isolated and perfused working eel (Anguilla anguilla) heart. Under basal conditions, the three peptides decreased stroke volume (SV) and stroke work (SW), thus exerting negative inotropism. The VS-1-mediated negative inotropism was abolished by exposure to inhibitors of either Gi/o protein (pertussis toxin; PTx) or M1 muscarinic receptors (Pirenzepine) or calcium (Lantanum and Diltiazem) and potassium (Ba 2+, 4-aminopyridine, tetraethylammonium, glibenclamide) channels, while it required an intact endocardial endothelium (EE). Using N G-monomethyl-l-arginine (l-NMMA) as an inhibitor of nitric oxide (NO) synthase (NOS), and hemoglobin as a NO scavenger, we demonstrated the obligatory role of NO signaling in mediating the vasostatin response. Pretreatment with either a specific inhibitor of soluble guanylate cyclase (GC) 1H-(1,2,4)oxadiazolo-(4,3-a)quinoxalin-1-one (ODQ), or the inhibitor of the cGMP-activated protein kinase (PKG) KT 5823, abolished the VS-1-mediated inotropism, indicating the cGMP-PKG component as a crucial target of NO signaling. Of note, VS-1 was effective in counteracting the adrenergic (Isoproterenol and Phenylephrine)-mediated positive inotropism. These findings provide the first evidence that vasostatins exert cardiotropic action in fish, thus suggesting their long evolutionary history as well as their species-specific mechanisms of action.",
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