Abstract
We previously reported that angiotensin-converting enzyme inhibitor (ACEi) renoprotection in Munich Wistar Frömter (MWF) rats, which develop progressive glomerular injury, was associated with podocyte repopulation and preservation of glomerular architecture. Here, we studied the time course of the lesions, their cellular components, and the effect of ACEi. Early glomerular lesions were synechiae, followed by extracapillary crescents and glomerulosclerosis. The majority of cells forming crescents were claudin1 + parietal epithelial cells and, to a lesser extent, WT1 + podocytes, both in active proliferation. In crescents, cells expressing the metanephric mesenchyme marker NCAM were also found. Three distinct populations of parietal epithelial cells were identified in the rat Bowman's capsule: NCAM +WT1 - cells, also expressing progenitor cell marker CD24, and NCAM +WT1 + and NCAM -WT1 + cells, the latter population representing parietal podocytes. After exposure to inductive medium, cultured parietal epithelial cells that were obtained by capsulated glomeruli generated podocytes, documenting their progenitor nature. Mitotic activity of cultured renal progenitors was induced by angiotensin II through the down-regulation of cell cycle inhibitor C/EBPδ expression. Treatment with ACEi reduced number and extension of crescents and glomerulosclerosis in MWF rats. Renoprotection was accomplished through the limitation of NCAM + progenitor proliferation via the modulation of C/EBPδ. Thus, chaotic migration and proliferation of the Bowman's capsule progenitor cells pave the way to crescent formation and subsequent sclerosis. ACEi, by moderating progenitor cell activation, restores glomerular architecture and prevents renal disease progression.
| Original language | English |
|---|---|
| Pages (from-to) | 628-638 |
| Number of pages | 11 |
| Journal | American Journal of Pathology |
| Volume | 179 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 2011 |
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ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Inhibiting angiotensin-converting enzyme promotes renal repair by limiting progenitor cell proliferation and restoring the glomerular architecture. / Benigni, Ariela; Morigi, Marina; Rizzo, Paola; Gagliardini, Elena; Rota, Cinzia; Abbate, Mauro; Ghezzi, Serena; Remuzzi, Andrea; Remuzzi, Giuseppe.
In: American Journal of Pathology, Vol. 179, No. 2, 08.2011, p. 628-638.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibiting angiotensin-converting enzyme promotes renal repair by limiting progenitor cell proliferation and restoring the glomerular architecture
AU - Benigni, Ariela
AU - Morigi, Marina
AU - Rizzo, Paola
AU - Gagliardini, Elena
AU - Rota, Cinzia
AU - Abbate, Mauro
AU - Ghezzi, Serena
AU - Remuzzi, Andrea
AU - Remuzzi, Giuseppe
PY - 2011/8
Y1 - 2011/8
N2 - We previously reported that angiotensin-converting enzyme inhibitor (ACEi) renoprotection in Munich Wistar Frömter (MWF) rats, which develop progressive glomerular injury, was associated with podocyte repopulation and preservation of glomerular architecture. Here, we studied the time course of the lesions, their cellular components, and the effect of ACEi. Early glomerular lesions were synechiae, followed by extracapillary crescents and glomerulosclerosis. The majority of cells forming crescents were claudin1 + parietal epithelial cells and, to a lesser extent, WT1 + podocytes, both in active proliferation. In crescents, cells expressing the metanephric mesenchyme marker NCAM were also found. Three distinct populations of parietal epithelial cells were identified in the rat Bowman's capsule: NCAM +WT1 - cells, also expressing progenitor cell marker CD24, and NCAM +WT1 + and NCAM -WT1 + cells, the latter population representing parietal podocytes. After exposure to inductive medium, cultured parietal epithelial cells that were obtained by capsulated glomeruli generated podocytes, documenting their progenitor nature. Mitotic activity of cultured renal progenitors was induced by angiotensin II through the down-regulation of cell cycle inhibitor C/EBPδ expression. Treatment with ACEi reduced number and extension of crescents and glomerulosclerosis in MWF rats. Renoprotection was accomplished through the limitation of NCAM + progenitor proliferation via the modulation of C/EBPδ. Thus, chaotic migration and proliferation of the Bowman's capsule progenitor cells pave the way to crescent formation and subsequent sclerosis. ACEi, by moderating progenitor cell activation, restores glomerular architecture and prevents renal disease progression.
AB - We previously reported that angiotensin-converting enzyme inhibitor (ACEi) renoprotection in Munich Wistar Frömter (MWF) rats, which develop progressive glomerular injury, was associated with podocyte repopulation and preservation of glomerular architecture. Here, we studied the time course of the lesions, their cellular components, and the effect of ACEi. Early glomerular lesions were synechiae, followed by extracapillary crescents and glomerulosclerosis. The majority of cells forming crescents were claudin1 + parietal epithelial cells and, to a lesser extent, WT1 + podocytes, both in active proliferation. In crescents, cells expressing the metanephric mesenchyme marker NCAM were also found. Three distinct populations of parietal epithelial cells were identified in the rat Bowman's capsule: NCAM +WT1 - cells, also expressing progenitor cell marker CD24, and NCAM +WT1 + and NCAM -WT1 + cells, the latter population representing parietal podocytes. After exposure to inductive medium, cultured parietal epithelial cells that were obtained by capsulated glomeruli generated podocytes, documenting their progenitor nature. Mitotic activity of cultured renal progenitors was induced by angiotensin II through the down-regulation of cell cycle inhibitor C/EBPδ expression. Treatment with ACEi reduced number and extension of crescents and glomerulosclerosis in MWF rats. Renoprotection was accomplished through the limitation of NCAM + progenitor proliferation via the modulation of C/EBPδ. Thus, chaotic migration and proliferation of the Bowman's capsule progenitor cells pave the way to crescent formation and subsequent sclerosis. ACEi, by moderating progenitor cell activation, restores glomerular architecture and prevents renal disease progression.
UR - http://www.scopus.com/inward/record.url?scp=80052499138&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052499138&partnerID=8YFLogxK
U2 - 10.1016/j.ajpath.2011.04.003
DO - 10.1016/j.ajpath.2011.04.003
M3 - Article
C2 - 21718676
AN - SCOPUS:80052499138
VL - 179
SP - 628
EP - 638
JO - American Journal of Pathology
JF - American Journal of Pathology
SN - 0002-9440
IS - 2
ER -