Inhibition of angiogenic activity of renal carcinoma by an antisense oligonucleotide targeting fibroblast growth factor-2

Elisabetta Cenni, Francesca Perut, Monia Zuntini, Donatella Granchi, Ilaria Amato, Sofia Avnet, Maria Luisa Brandi, Armando Giunti, Nicola Baldini

Research output: Contribution to journalArticle

Abstract

Background: Fibroblast growth factor-2 (FGF-2) induces angiogenesis, critical for the growth and metastatic spread of tumors. Materials and Methods: The effect of blocking FGF-2 synthesis by an antisense phosphorothioate oligodeoxynucleotide (PS-ODN2) was evaluated on the angiogenic activity of Caki-1 and of a cell line isolated from a renal carcinoma bone metastasis (CRBM-1990). After the transfection with PS-ODN2, FGF-2 mRNA, protein expression and angiogenic activity were evaluated. Results: In Caki-1, a not significant decrease in the released FGF-2 was observed after 72 hours. In CRBM-1990, a not significant decrease in intracellular FGF-2 protein was observed after 72 hours. Endothelial cell migration induced by the conditioned media from Caki-1 treated with PS-ODN2 for 72 hours was significantly reduced. Conclusion: PS-ODN2 treatment of the established line Caki-1 induced minimal variations in FGF-2 expression, but inhibited endothelial cell migration. In CRBM-1990 cells, PS-ODN2 determined a decrease in intracellular protein without reducing the ability to induce endothelial cell migration and proliferation.

Original languageEnglish
Pages (from-to)1109-1113
Number of pages5
JournalAnticancer Research
Volume25
Issue number2 A
Publication statusPublished - Mar 2005

Keywords

  • Angiogenesis
  • Antisense oligodeoxynucleotide
  • Bone metastasis
  • Fibroblast growth factor-2
  • Renal cell carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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