Inhibition of endothelial cell migration and angiogenesis by a vascular endothelial growth factor receptor-1 derived peptide

Pedro M. Lacal, Veronica Morea, Federica Ruffini, Angela Orecchia, Annalisa S. Dorio, Cristina M. Failla, Simonetta Soro, Lucio Tentori, Giovanna Zambruno, Grazia Graziani, Anna Tramontano, Stefania D'Atri

Research output: Contribution to journalArticle


Vascular endothelial growth factor receptor-1 (VEGFR-1) exists in two isoforms: a membrane-bound isoform (mVEGFR-1) and a soluble one (sVEGFR-1). mVEGFR-1 is involved in endothelial cell migration and survival supported by VEGF-A and placenta growth factor (PlGF), whereas the biologic function of sVEGFR-1 has not been fully elucidated. We previously reported that sVEGFR-1 induces endothelial cell motility and promotes endothelial cell adhesion. In this study, we tested a set of VEGFR-1-derived peptides for their ability to interfere with endothelial cell migration. Peptide B3 was found to specifically inhibit cell migration induced by sVEGFR-1 and by mVEGFR-1-specific ligands. Moreover, peptide B3 markedly hampered angiogenesis in vitro and in vivo and was found to interfere with VEGFR-1 homodimerisation. Altogether, these data demonstrate that peptide B3 might be a useful tool for the specific inhibition of VEGFR-1 function and might represent a basis for the development of new anti-angiogenic compounds.

Original languageEnglish
Pages (from-to)1914-1921
Number of pages8
JournalEuropean Journal of Cancer
Issue number13
Publication statusPublished - Sep 2008



  • Angiogenesis
  • Endothelial cells
  • Migration
  • PlGF
  • Soluble VEGFR-1
  • VEGFR-1 activation
  • VEGFR-1 peptides

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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