Inhibition of glutamate-induced neurotoxicity by a tau antisense oligonucleotide in primary culture of rat cerebellar granule cells

M. Pizzi, A. Valerio, V. Arrighi, P. Galli, M. Belloni, M. Ribola, A. Alberici, P. F. Spano, M. Memo

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Short-term exposure of primary cultures of cerebellar granule cells from neonatal rat brain to high concentrations of glutamate resulted in a significant increase of both immunoreactivity to and mRNA levels of tau protein. Time-course experiments revealed the increases of tau immunoreactivity and mRNA levels to be maximal 2 h after the glutamate pulse. To investigate the relationship between newly synthesized tau protein and glutamate-induced neurotoxicity, neurons were preincubated with a specific tau antisense oligonucleotide. This treatment resulted in (i) inhibition of the glutamate-induced increase of tau immunoreactivity and (ii) a decrease in the sensitivity of the neurons to neurotoxic concentrations of glutamate. These data indicate that induction of the cytoskeleton-associated tau protein participates in the cascade of events promoted by glutamate leading to neurodegeneration.

Original languageEnglish
Pages (from-to)1603-1613
Number of pages11
JournalEuropean Journal of Neuroscience
Volume7
Issue number7
DOIs
Publication statusPublished - 1995

Fingerprint

Antisense Oligonucleotides
Glutamic Acid
tau Proteins
Neurons
Messenger RNA
Cytoskeleton
Brain

Keywords

  • Cytoskeleton
  • Excitatory aminoacids
  • Neurodegeneration
  • Phosphorylation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Inhibition of glutamate-induced neurotoxicity by a tau antisense oligonucleotide in primary culture of rat cerebellar granule cells. / Pizzi, M.; Valerio, A.; Arrighi, V.; Galli, P.; Belloni, M.; Ribola, M.; Alberici, A.; Spano, P. F.; Memo, M.

In: European Journal of Neuroscience, Vol. 7, No. 7, 1995, p. 1603-1613.

Research output: Contribution to journalArticle

Pizzi, M, Valerio, A, Arrighi, V, Galli, P, Belloni, M, Ribola, M, Alberici, A, Spano, PF & Memo, M 1995, 'Inhibition of glutamate-induced neurotoxicity by a tau antisense oligonucleotide in primary culture of rat cerebellar granule cells', European Journal of Neuroscience, vol. 7, no. 7, pp. 1603-1613. https://doi.org/10.1111/j.1460-9568.1995.tb01156.x
Pizzi, M. ; Valerio, A. ; Arrighi, V. ; Galli, P. ; Belloni, M. ; Ribola, M. ; Alberici, A. ; Spano, P. F. ; Memo, M. / Inhibition of glutamate-induced neurotoxicity by a tau antisense oligonucleotide in primary culture of rat cerebellar granule cells. In: European Journal of Neuroscience. 1995 ; Vol. 7, No. 7. pp. 1603-1613.
@article{ada75fc4d0c640e1b10e0952b27c888d,
title = "Inhibition of glutamate-induced neurotoxicity by a tau antisense oligonucleotide in primary culture of rat cerebellar granule cells",
abstract = "Short-term exposure of primary cultures of cerebellar granule cells from neonatal rat brain to high concentrations of glutamate resulted in a significant increase of both immunoreactivity to and mRNA levels of tau protein. Time-course experiments revealed the increases of tau immunoreactivity and mRNA levels to be maximal 2 h after the glutamate pulse. To investigate the relationship between newly synthesized tau protein and glutamate-induced neurotoxicity, neurons were preincubated with a specific tau antisense oligonucleotide. This treatment resulted in (i) inhibition of the glutamate-induced increase of tau immunoreactivity and (ii) a decrease in the sensitivity of the neurons to neurotoxic concentrations of glutamate. These data indicate that induction of the cytoskeleton-associated tau protein participates in the cascade of events promoted by glutamate leading to neurodegeneration.",
keywords = "Cytoskeleton, Excitatory aminoacids, Neurodegeneration, Phosphorylation",
author = "M. Pizzi and A. Valerio and V. Arrighi and P. Galli and M. Belloni and M. Ribola and A. Alberici and Spano, {P. F.} and M. Memo",
year = "1995",
doi = "10.1111/j.1460-9568.1995.tb01156.x",
language = "English",
volume = "7",
pages = "1603--1613",
journal = "European Journal of Neuroscience",
issn = "0953-816X",
publisher = "Wiley-Blackwell",
number = "7",

}

TY - JOUR

T1 - Inhibition of glutamate-induced neurotoxicity by a tau antisense oligonucleotide in primary culture of rat cerebellar granule cells

AU - Pizzi, M.

AU - Valerio, A.

AU - Arrighi, V.

AU - Galli, P.

AU - Belloni, M.

AU - Ribola, M.

AU - Alberici, A.

AU - Spano, P. F.

AU - Memo, M.

PY - 1995

Y1 - 1995

N2 - Short-term exposure of primary cultures of cerebellar granule cells from neonatal rat brain to high concentrations of glutamate resulted in a significant increase of both immunoreactivity to and mRNA levels of tau protein. Time-course experiments revealed the increases of tau immunoreactivity and mRNA levels to be maximal 2 h after the glutamate pulse. To investigate the relationship between newly synthesized tau protein and glutamate-induced neurotoxicity, neurons were preincubated with a specific tau antisense oligonucleotide. This treatment resulted in (i) inhibition of the glutamate-induced increase of tau immunoreactivity and (ii) a decrease in the sensitivity of the neurons to neurotoxic concentrations of glutamate. These data indicate that induction of the cytoskeleton-associated tau protein participates in the cascade of events promoted by glutamate leading to neurodegeneration.

AB - Short-term exposure of primary cultures of cerebellar granule cells from neonatal rat brain to high concentrations of glutamate resulted in a significant increase of both immunoreactivity to and mRNA levels of tau protein. Time-course experiments revealed the increases of tau immunoreactivity and mRNA levels to be maximal 2 h after the glutamate pulse. To investigate the relationship between newly synthesized tau protein and glutamate-induced neurotoxicity, neurons were preincubated with a specific tau antisense oligonucleotide. This treatment resulted in (i) inhibition of the glutamate-induced increase of tau immunoreactivity and (ii) a decrease in the sensitivity of the neurons to neurotoxic concentrations of glutamate. These data indicate that induction of the cytoskeleton-associated tau protein participates in the cascade of events promoted by glutamate leading to neurodegeneration.

KW - Cytoskeleton

KW - Excitatory aminoacids

KW - Neurodegeneration

KW - Phosphorylation

UR - http://www.scopus.com/inward/record.url?scp=0029043272&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029043272&partnerID=8YFLogxK

U2 - 10.1111/j.1460-9568.1995.tb01156.x

DO - 10.1111/j.1460-9568.1995.tb01156.x

M3 - Article

C2 - 7551187

AN - SCOPUS:0029043272

VL - 7

SP - 1603

EP - 1613

JO - European Journal of Neuroscience

JF - European Journal of Neuroscience

SN - 0953-816X

IS - 7

ER -