Inhibition of growth of breast cancer cells in vitro by the ribosome-inactivating protein Saporin 6

A. Gasperi-Campani, W. Zoli, A. Volpi, L. Roncuzzi, D. Amadori

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The antiproliferative activity of Saporin 6, a Ribosome-inactivating protein purified from the seeds of Saponaria officinalis has been tested on human breast cancer cells in vitro by the analysis (a) of colony formation in cells from surgical specimens from 27 patients bearing primary breast cancer and (b) of protein synthesis inhibition in the MCF/7 cell line. Results indicate a very high sensitivity of breast cancer cells from most patients to a short-term treatment with Saporin 6 at concentrations (10-9 M), until now found effective only in acellular systems or after conjugation with monoclonal antibodies. On the contrary, the treatment of the human cell line MCF/7 indicate a very reduced sensitivity compared to fresh human neoplastic cells, with the necessity of a long lag for the effect to begin.

Original languageEnglish
Pages (from-to)1007-1011
Number of pages5
JournalAnticancer Research
Volume11
Issue number2
Publication statusPublished - 1991

Fingerprint

Ribosome Inactivating Proteins
Breast Neoplasms
Growth
Saponaria
Cell Line
MCF-7 Cells
Seeds
Monoclonal Antibodies
In Vitro Techniques
saporin
Therapeutics
Proteins

Keywords

  • antiproliferative effect
  • human breast cancer
  • Saporin 6

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Inhibition of growth of breast cancer cells in vitro by the ribosome-inactivating protein Saporin 6. / Gasperi-Campani, A.; Zoli, W.; Volpi, A.; Roncuzzi, L.; Amadori, D.

In: Anticancer Research, Vol. 11, No. 2, 1991, p. 1007-1011.

Research output: Contribution to journalArticle

Gasperi-Campani, A, Zoli, W, Volpi, A, Roncuzzi, L & Amadori, D 1991, 'Inhibition of growth of breast cancer cells in vitro by the ribosome-inactivating protein Saporin 6', Anticancer Research, vol. 11, no. 2, pp. 1007-1011.
Gasperi-Campani, A. ; Zoli, W. ; Volpi, A. ; Roncuzzi, L. ; Amadori, D. / Inhibition of growth of breast cancer cells in vitro by the ribosome-inactivating protein Saporin 6. In: Anticancer Research. 1991 ; Vol. 11, No. 2. pp. 1007-1011.
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