Inhibition of human platelet aggregation by plasmin digests of human and bovine fibrinogen preparations: role of contaminating factor VIII related material

D. E. Culasso, M. B. Donati, G. de Gaetano, J. Vermylen, M. Verstraete

Research output: Contribution to journalArticlepeer-review

Abstract

Preparations of human fibrinogen, digested by plasmin, inhibited ADP induced platelet aggregation; the inhibitory activity was confined to the small dialyzable fragments accumulating during the degradation. Purified large molecular weight fragments D and E had no effect on ADP induced aggregation, but fragment E inhibited thrombin induced aggregation. Extensively degraded bovine fibrinogen preparations also inhibited platelet aggregation by ADP. Both human and bovine fibrinogen preparations were contaminated with factor VIII related material (factor VIII related antigen and factor VIII procoagulant activity, respectively); separation of factor VIII related material from human or bovine fibrinogen by gel chromatography and subsequent plasmin digestion of the fractions revealed that the inhibitory activity was mainly linked to digested factor VIII related material. This inhibitory activity was dialyzable. The effect of fibrinogen digests on platelet aggregation should therefore be reconsidered.

Original languageEnglish
Pages (from-to)169-175
Number of pages7
JournalBlood
Volume44
Issue number2
Publication statusPublished - 1974

ASJC Scopus subject areas

  • Hematology

Fingerprint

Dive into the research topics of 'Inhibition of human platelet aggregation by plasmin digests of human and bovine fibrinogen preparations: role of contaminating factor VIII related material'. Together they form a unique fingerprint.

Cite this