Inhibition of in vitro growth of enteropathogens by new Lactobacillus isolates of human intestinal origin

Lorenzo Drago, Maria Rita Gismondo, Alessandra Lombardi, Christoph De Haën, Luigia Gozzini

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Three human Lactobacillus strains, coded B21060, B21070 and B21190, have recently been isolated. The strains show a series of features (acid and bile resistance, adhesion to various types of mucosal cell) which make them particularly promising for the preparation of probiotic products. In the present study, the ability of the strains to inhibit the growth of pathogens in coculture was investigated. Lactobacilli were incubated simultaneously or after one overnight growth with enterotoxigenic Escherichia coli, Salmonella enteritidis or Vibrio cholerae. After 24 and 48 h, bacterial counts of the pathogens and of the lactobacilli were performed. The results showed that these Lactobacillus strains inhibited the in vitro growth of E. coli and S. enteritidis under both conditions. Moreover, a cumulative effect was observed for mixtures of lactobacilli. In contrast, no significant inhibition of Vibrio cholerae growth was observed, provided that the pH of the medium was kept constant. The presence of the pathogens did not affect the growth of the Lactobacillus strains. Moreover, each of the Lactobacillus strains showed coaggregation ability with two pathogenic E. coli strains, namely ATCC 25922 and ATCC 35401.

Original languageEnglish
Pages (from-to)455-463
Number of pages9
JournalFEMS Microbiology Letters
Volume153
Issue number2
DOIs
Publication statusPublished - Aug 15 1997

Fingerprint

Lactobacillus
Growth
Salmonella enteritidis
Vibrio cholerae
Escherichia coli
Enterotoxigenic Escherichia coli
Bacterial Load
Probiotics
Coculture Techniques
In Vitro Techniques
Bile Acids and Salts

Keywords

  • Bacterial coaggregation
  • Enteropathogen
  • Growth inhibition
  • New human Lactobacillus strain

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Inhibition of in vitro growth of enteropathogens by new Lactobacillus isolates of human intestinal origin. / Drago, Lorenzo; Gismondo, Maria Rita; Lombardi, Alessandra; De Haën, Christoph; Gozzini, Luigia.

In: FEMS Microbiology Letters, Vol. 153, No. 2, 15.08.1997, p. 455-463.

Research output: Contribution to journalArticle

Drago, Lorenzo ; Gismondo, Maria Rita ; Lombardi, Alessandra ; De Haën, Christoph ; Gozzini, Luigia. / Inhibition of in vitro growth of enteropathogens by new Lactobacillus isolates of human intestinal origin. In: FEMS Microbiology Letters. 1997 ; Vol. 153, No. 2. pp. 455-463.
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