Inhibition of in vitro myogenic differentiation by a polyomavirus early function

R. Maione, G. M. Fimia, P. Amati

Research output: Contribution to journalArticlepeer-review

Abstract

In the present work we report on the role of a polyomavirus (Py) early function in interfering with both morphological and biochemical differentiation of the myogenic C2 cell line. The analysis of cell clones stably transfected with a plasmid carrying an ORI- Py genome showed that in the presence of the whole viral early region myogenesis is blocked and a transformed phenotype is evident. By using a plasmid that only encodes large-T function, the involvement of this individual early viral gene product was determined. Inhibition of myogenic differentiation by Py large T is proportional to the level of its expression. This inhibition does not appear to require alteration of cell growth properties. The analysis of muscle-specific functions expressed at different steps in the myogenic pathway showed that Py large T blocks the expression of terminal differentiation markers without altering the expression of the regulatory gene MyoD.

Original languageEnglish
Pages (from-to)85-93
Number of pages9
JournalOncogene
Volume7
Issue number1
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

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