Abstract
In the present work we report on the role of a polyomavirus (Py) early function in interfering with both morphological and biochemical differentiation of the myogenic C2 cell line. The analysis of cell clones stably transfected with a plasmid carrying an ORI- Py genome showed that in the presence of the whole viral early region myogenesis is blocked and a transformed phenotype is evident. By using a plasmid that only encodes large-T function, the involvement of this individual early viral gene product was determined. Inhibition of myogenic differentiation by Py large T is proportional to the level of its expression. This inhibition does not appear to require alteration of cell growth properties. The analysis of muscle-specific functions expressed at different steps in the myogenic pathway showed that Py large T blocks the expression of terminal differentiation markers without altering the expression of the regulatory gene MyoD.
Original language | English |
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Pages (from-to) | 85-93 |
Number of pages | 9 |
Journal | Oncogene |
Volume | 7 |
Issue number | 1 |
Publication status | Published - 1992 |
ASJC Scopus subject areas
- Cancer Research
- Genetics
- Molecular Biology