Inhibition of inducible nitric oxide synthase expression by an acetonic extract from Feijoa sellowiana Berg. fruits

Antonietta Rossi, Daniela Rigano, Carlo Pergola, Carmen Formisano, Adriana Basile, Placido Bramanti, Felice Senatore, Lidia Sautebin

Research output: Contribution to journalArticle

Abstract

Feijoa sellowiana Berg. fruits and especially the acetonic extract have been shown to possess biological activities, although the responsible compounds have never been identified. The present study was designed to evaluate the anti-inflammatory activity of an acetonic extract from F. sellowiana Berg. fruits on the nitric oxide (NO) pathway, which plays an important role in inflammation. To this aim the J774 cell line, which expresses inducible nitric oxide synthase (iNOS) following stimulation with lipopolysaccharide (LPS), has been utilized, and the effects of this extract and its fractions on NO production, iNOS protein expression, and signal pathways involved in its regulation have been evaluated. This study demonstrates that at least some part of the anti-inflammatory activity of the acetonic extract is due to the suppression of NO production by flavone and stearic acid. The mechanism of this inhibition seems to be related to an action on the expression of the enzyme iNOS through the attenuation of nuclear factor κB (NF-κB) and/or mitogen-activated protein kinase (MAPK) activation.

Original languageEnglish
Pages (from-to)5053-5061
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume55
Issue number13
DOIs
Publication statusPublished - Jun 27 2007

Fingerprint

Feijoa
Acca sellowiana
Nitric Oxide Synthase Type II
Fruits
Fruit
Nitric Oxide
flavone
nitric oxide
fruits
extracts
Anti-Inflammatory Agents
anti-inflammatory activity
Bioactivity
Mitogen-Activated Protein Kinases
Lipopolysaccharides
Signal Transduction
flavones
stearic acid
Chemical activation
mitogen-activated protein kinase

Keywords

  • ERK-1/2
  • Fatty acid
  • Feijoa
  • Feijoa sellowiana
  • Flavone
  • iNOS
  • Macrophages
  • NF-κB

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Inhibition of inducible nitric oxide synthase expression by an acetonic extract from Feijoa sellowiana Berg. fruits. / Rossi, Antonietta; Rigano, Daniela; Pergola, Carlo; Formisano, Carmen; Basile, Adriana; Bramanti, Placido; Senatore, Felice; Sautebin, Lidia.

In: Journal of Agricultural and Food Chemistry, Vol. 55, No. 13, 27.06.2007, p. 5053-5061.

Research output: Contribution to journalArticle

Rossi, Antonietta ; Rigano, Daniela ; Pergola, Carlo ; Formisano, Carmen ; Basile, Adriana ; Bramanti, Placido ; Senatore, Felice ; Sautebin, Lidia. / Inhibition of inducible nitric oxide synthase expression by an acetonic extract from Feijoa sellowiana Berg. fruits. In: Journal of Agricultural and Food Chemistry. 2007 ; Vol. 55, No. 13. pp. 5053-5061.
@article{d3385228c784459199b13ffde83a6cf6,
title = "Inhibition of inducible nitric oxide synthase expression by an acetonic extract from Feijoa sellowiana Berg. fruits",
abstract = "Feijoa sellowiana Berg. fruits and especially the acetonic extract have been shown to possess biological activities, although the responsible compounds have never been identified. The present study was designed to evaluate the anti-inflammatory activity of an acetonic extract from F. sellowiana Berg. fruits on the nitric oxide (NO) pathway, which plays an important role in inflammation. To this aim the J774 cell line, which expresses inducible nitric oxide synthase (iNOS) following stimulation with lipopolysaccharide (LPS), has been utilized, and the effects of this extract and its fractions on NO production, iNOS protein expression, and signal pathways involved in its regulation have been evaluated. This study demonstrates that at least some part of the anti-inflammatory activity of the acetonic extract is due to the suppression of NO production by flavone and stearic acid. The mechanism of this inhibition seems to be related to an action on the expression of the enzyme iNOS through the attenuation of nuclear factor κB (NF-κB) and/or mitogen-activated protein kinase (MAPK) activation.",
keywords = "ERK-1/2, Fatty acid, Feijoa, Feijoa sellowiana, Flavone, iNOS, Macrophages, NF-κB",
author = "Antonietta Rossi and Daniela Rigano and Carlo Pergola and Carmen Formisano and Adriana Basile and Placido Bramanti and Felice Senatore and Lidia Sautebin",
year = "2007",
month = "6",
day = "27",
doi = "10.1021/jf070510d",
language = "English",
volume = "55",
pages = "5053--5061",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "13",

}

TY - JOUR

T1 - Inhibition of inducible nitric oxide synthase expression by an acetonic extract from Feijoa sellowiana Berg. fruits

AU - Rossi, Antonietta

AU - Rigano, Daniela

AU - Pergola, Carlo

AU - Formisano, Carmen

AU - Basile, Adriana

AU - Bramanti, Placido

AU - Senatore, Felice

AU - Sautebin, Lidia

PY - 2007/6/27

Y1 - 2007/6/27

N2 - Feijoa sellowiana Berg. fruits and especially the acetonic extract have been shown to possess biological activities, although the responsible compounds have never been identified. The present study was designed to evaluate the anti-inflammatory activity of an acetonic extract from F. sellowiana Berg. fruits on the nitric oxide (NO) pathway, which plays an important role in inflammation. To this aim the J774 cell line, which expresses inducible nitric oxide synthase (iNOS) following stimulation with lipopolysaccharide (LPS), has been utilized, and the effects of this extract and its fractions on NO production, iNOS protein expression, and signal pathways involved in its regulation have been evaluated. This study demonstrates that at least some part of the anti-inflammatory activity of the acetonic extract is due to the suppression of NO production by flavone and stearic acid. The mechanism of this inhibition seems to be related to an action on the expression of the enzyme iNOS through the attenuation of nuclear factor κB (NF-κB) and/or mitogen-activated protein kinase (MAPK) activation.

AB - Feijoa sellowiana Berg. fruits and especially the acetonic extract have been shown to possess biological activities, although the responsible compounds have never been identified. The present study was designed to evaluate the anti-inflammatory activity of an acetonic extract from F. sellowiana Berg. fruits on the nitric oxide (NO) pathway, which plays an important role in inflammation. To this aim the J774 cell line, which expresses inducible nitric oxide synthase (iNOS) following stimulation with lipopolysaccharide (LPS), has been utilized, and the effects of this extract and its fractions on NO production, iNOS protein expression, and signal pathways involved in its regulation have been evaluated. This study demonstrates that at least some part of the anti-inflammatory activity of the acetonic extract is due to the suppression of NO production by flavone and stearic acid. The mechanism of this inhibition seems to be related to an action on the expression of the enzyme iNOS through the attenuation of nuclear factor κB (NF-κB) and/or mitogen-activated protein kinase (MAPK) activation.

KW - ERK-1/2

KW - Fatty acid

KW - Feijoa

KW - Feijoa sellowiana

KW - Flavone

KW - iNOS

KW - Macrophages

KW - NF-κB

UR - http://www.scopus.com/inward/record.url?scp=34447345966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447345966&partnerID=8YFLogxK

U2 - 10.1021/jf070510d

DO - 10.1021/jf070510d

M3 - Article

C2 - 17550270

AN - SCOPUS:34447345966

VL - 55

SP - 5053

EP - 5061

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 13

ER -