Inhibition of inducible nitric oxide synthase expression by an acetonic extract from Feijoa sellowiana Berg. fruits

Antonietta Rossi, Daniela Rigano, Carlo Pergola, Carmen Formisano, Adriana Basile, Placido Bramanti, Felice Senatore, Lidia Sautebin

Research output: Contribution to journalArticlepeer-review

Abstract

Feijoa sellowiana Berg. fruits and especially the acetonic extract have been shown to possess biological activities, although the responsible compounds have never been identified. The present study was designed to evaluate the anti-inflammatory activity of an acetonic extract from F. sellowiana Berg. fruits on the nitric oxide (NO) pathway, which plays an important role in inflammation. To this aim the J774 cell line, which expresses inducible nitric oxide synthase (iNOS) following stimulation with lipopolysaccharide (LPS), has been utilized, and the effects of this extract and its fractions on NO production, iNOS protein expression, and signal pathways involved in its regulation have been evaluated. This study demonstrates that at least some part of the anti-inflammatory activity of the acetonic extract is due to the suppression of NO production by flavone and stearic acid. The mechanism of this inhibition seems to be related to an action on the expression of the enzyme iNOS through the attenuation of nuclear factor κB (NF-κB) and/or mitogen-activated protein kinase (MAPK) activation.

Original languageEnglish
Pages (from-to)5053-5061
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume55
Issue number13
DOIs
Publication statusPublished - Jun 27 2007

Keywords

  • ERK-1/2
  • Fatty acid
  • Feijoa
  • Feijoa sellowiana
  • Flavone
  • iNOS
  • Macrophages
  • NF-κB

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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