Inhibition of inflammatory angiogenesis in rats by loco-regional administration of hydrocortisone and protamine

D. Foschi, L. Castoldi, F. Corsi, E. Radaelli, E. Trabucchi

Research output: Contribution to journalArticle

Abstract

We have studied the antiangiogenetic effects of hydrocortisone and protamine given intra-arterially. The cornea of male, Sprague-Dawley rats were cauterized with silver nitrate. The following treatments were given :30 μg hydrocortisone topical (t.p.), b.i.d., 50 mg/kg/day intraperitoneally (i.p.) or intra-arterially (i.a.), 10 mg/ kg/day protamine i.p. or i.a. Saline was administered to the control groups. In separate experiments we also evaluated the anti-inflammatory effects of hydrocortisone, i.p., on the cauterized corneas. Five days after cauterization, the animals were killed, exsanguinated and India ink was injected to show the network of neovessels. The percentage area of the cornea covered by neovessels was measured morphometrically and evaluated statistically. Hydrocortisone t.p. (-84%), i.a. (-60%) and protamine i.a. (-44%) significantly inhibited angiogenesis in the cauterized cornea. Either drugs, i.p., had any antiangiogenetic effects, but hydrocortisone significantly reduced cell infiltration of the corneas. The results suggest that locoregional administration of antiangiogenetic drugs might be clinically useful.

Original languageEnglish
Pages (from-to)40-43
Number of pages4
JournalAgents and Actions
Volume42
Issue number1-2
DOIs
Publication statusPublished - Aug 1994

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Keywords

  • Angiogenesis
  • Corticosteroids
  • Inflammation
  • Protamine
  • Vessels

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)

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