Inhibition of lipoxygenase in lentil protoplasts by expression of antisense RNA

Mauro Maccarrone; Martin P. Hilbers; Gerrit A. Veldink; Johannes F G Vliegenthart; Alessandro Finazzi Agrò

doi:10.1016/0005-2760(95)00153-4

Inhibition of lipoxygenase in lentil protoplasts by expression of antisense RNA

Mauro Maccarrone, Martin P. Hilbers, Gerrit A. Veldink, Johannes F G Vliegenthart, Alessandro Finazzi Agrò

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

A number of plasmids were constructed containing chimeric genes consisting of fragments of antisense-oriented lentil lipoxygenase cDNA. The different constructs were tested for their ability to lower lipoxygenase activity in lentil protoplasts. Plasmids containing a full length lentil lipoxygenase cDNA proved to be the most effective, reducing the activity of the target enzyme to 70% of the control value. On the other hand, the full length lentil lipoxygenase cDNA in the sense orientation yielded a 20% increase of lipoxygenase activity.

Original language	English
Pages (from-to)	1-3
Number of pages	3
Journal	Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume	1259
Issue number	1
DOIs	https://doi.org/10.1016/0005-2760(95)00153-4
Publication status	Published - Oct 26 1995

Keywords

Antisense RNA
Electroporation
Lentil (Lens culinaris)
Lipoxygenase
Protoplast

ASJC Scopus subject areas

Biochemistry
Biophysics
Endocrinology

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10.1016/0005-2760(95)00153-4

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Inhibition of lipoxygenase in lentil protoplasts by expression of antisense RNA. / Maccarrone, Mauro; Hilbers, Martin P.; Veldink, Gerrit A.; Vliegenthart, Johannes F G; Agrò, Alessandro Finazzi.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 1259, No. 1, 26.10.1995, p. 1-3.

Research output: Contribution to journal › Article › peer-review

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abstract = "A number of plasmids were constructed containing chimeric genes consisting of fragments of antisense-oriented lentil lipoxygenase cDNA. The different constructs were tested for their ability to lower lipoxygenase activity in lentil protoplasts. Plasmids containing a full length lentil lipoxygenase cDNA proved to be the most effective, reducing the activity of the target enzyme to 70% of the control value. On the other hand, the full length lentil lipoxygenase cDNA in the sense orientation yielded a 20% increase of lipoxygenase activity.",

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AU - Agrò, Alessandro Finazzi

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N2 - A number of plasmids were constructed containing chimeric genes consisting of fragments of antisense-oriented lentil lipoxygenase cDNA. The different constructs were tested for their ability to lower lipoxygenase activity in lentil protoplasts. Plasmids containing a full length lentil lipoxygenase cDNA proved to be the most effective, reducing the activity of the target enzyme to 70% of the control value. On the other hand, the full length lentil lipoxygenase cDNA in the sense orientation yielded a 20% increase of lipoxygenase activity.

AB - A number of plasmids were constructed containing chimeric genes consisting of fragments of antisense-oriented lentil lipoxygenase cDNA. The different constructs were tested for their ability to lower lipoxygenase activity in lentil protoplasts. Plasmids containing a full length lentil lipoxygenase cDNA proved to be the most effective, reducing the activity of the target enzyme to 70% of the control value. On the other hand, the full length lentil lipoxygenase cDNA in the sense orientation yielded a 20% increase of lipoxygenase activity.

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KW - Electroporation

KW - Lentil (Lens culinaris)

KW - Lipoxygenase

KW - Protoplast

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Inhibition of lipoxygenase in lentil protoplasts by expression of antisense RNA

Abstract

Keywords

ASJC Scopus subject areas

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