Inhibition of lipoxygenase in lentil protoplasts by expression of antisense RNA

Mauro Maccarrone, Martin P. Hilbers, Gerrit A. Veldink, Johannes F G Vliegenthart, Alessandro Finazzi Agrò

Research output: Contribution to journalArticlepeer-review

Abstract

A number of plasmids were constructed containing chimeric genes consisting of fragments of antisense-oriented lentil lipoxygenase cDNA. The different constructs were tested for their ability to lower lipoxygenase activity in lentil protoplasts. Plasmids containing a full length lentil lipoxygenase cDNA proved to be the most effective, reducing the activity of the target enzyme to 70% of the control value. On the other hand, the full length lentil lipoxygenase cDNA in the sense orientation yielded a 20% increase of lipoxygenase activity.

Original languageEnglish
Pages (from-to)1-3
Number of pages3
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume1259
Issue number1
DOIs
Publication statusPublished - Oct 26 1995

Keywords

  • Antisense RNA
  • Electroporation
  • Lentil (Lens culinaris)
  • Lipoxygenase
  • Protoplast

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology

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