Abstract
A number of plasmids were constructed containing chimeric genes consisting of fragments of antisense-oriented lentil lipoxygenase cDNA. The different constructs were tested for their ability to lower lipoxygenase activity in lentil protoplasts. Plasmids containing a full length lentil lipoxygenase cDNA proved to be the most effective, reducing the activity of the target enzyme to 70% of the control value. On the other hand, the full length lentil lipoxygenase cDNA in the sense orientation yielded a 20% increase of lipoxygenase activity.
| Original language | English |
|---|---|
| Pages (from-to) | 1-3 |
| Number of pages | 3 |
| Journal | Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism |
| Volume | 1259 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Oct 26 1995 |
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Keywords
- Antisense RNA
- Electroporation
- Lentil (Lens culinaris)
- Lipoxygenase
- Protoplast
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Endocrinology
Cite this
Inhibition of lipoxygenase in lentil protoplasts by expression of antisense RNA. / Maccarrone, Mauro; Hilbers, Martin P.; Veldink, Gerrit A.; Vliegenthart, Johannes F G; Agrò, Alessandro Finazzi.
In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 1259, No. 1, 26.10.1995, p. 1-3.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of lipoxygenase in lentil protoplasts by expression of antisense RNA
AU - Maccarrone, Mauro
AU - Hilbers, Martin P.
AU - Veldink, Gerrit A.
AU - Vliegenthart, Johannes F G
AU - Agrò, Alessandro Finazzi
PY - 1995/10/26
Y1 - 1995/10/26
N2 - A number of plasmids were constructed containing chimeric genes consisting of fragments of antisense-oriented lentil lipoxygenase cDNA. The different constructs were tested for their ability to lower lipoxygenase activity in lentil protoplasts. Plasmids containing a full length lentil lipoxygenase cDNA proved to be the most effective, reducing the activity of the target enzyme to 70% of the control value. On the other hand, the full length lentil lipoxygenase cDNA in the sense orientation yielded a 20% increase of lipoxygenase activity.
AB - A number of plasmids were constructed containing chimeric genes consisting of fragments of antisense-oriented lentil lipoxygenase cDNA. The different constructs were tested for their ability to lower lipoxygenase activity in lentil protoplasts. Plasmids containing a full length lentil lipoxygenase cDNA proved to be the most effective, reducing the activity of the target enzyme to 70% of the control value. On the other hand, the full length lentil lipoxygenase cDNA in the sense orientation yielded a 20% increase of lipoxygenase activity.
KW - Antisense RNA
KW - Electroporation
KW - Lentil (Lens culinaris)
KW - Lipoxygenase
KW - Protoplast
UR - http://www.scopus.com/inward/record.url?scp=0028811603&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028811603&partnerID=8YFLogxK
U2 - 10.1016/0005-2760(95)00153-4
DO - 10.1016/0005-2760(95)00153-4
M3 - Article
C2 - 7492607
AN - SCOPUS:0028811603
VL - 1259
SP - 1
EP - 3
JO - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism
JF - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism
SN - 0005-2760
IS - 1
ER -