Abstract
A number of plasmids were constructed containing chimeric genes consisting of fragments of antisense-oriented lentil lipoxygenase cDNA. The different constructs were tested for their ability to lower lipoxygenase activity in lentil protoplasts. Plasmids containing a full length lentil lipoxygenase cDNA proved to be the most effective, reducing the activity of the target enzyme to 70% of the control value. On the other hand, the full length lentil lipoxygenase cDNA in the sense orientation yielded a 20% increase of lipoxygenase activity.
Original language | English |
---|---|
Pages (from-to) | 1-3 |
Number of pages | 3 |
Journal | Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism |
Volume | 1259 |
Issue number | 1 |
DOIs | |
Publication status | Published - Oct 26 1995 |
Keywords
- Antisense RNA
- Electroporation
- Lentil (Lens culinaris)
- Lipoxygenase
- Protoplast
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Endocrinology