Inhibition of lipoxygenase in lentil protoplasts by expression of antisense RNA

Mauro Maccarrone, Martin P. Hilbers, Gerrit A. Veldink, Johannes F G Vliegenthart, Alessandro Finazzi Agrò

Research output: Contribution to journal › Article

Abstract

A number of plasmids were constructed containing chimeric genes consisting of fragments of antisense-oriented lentil lipoxygenase cDNA. The different constructs were tested for their ability to lower lipoxygenase activity in lentil protoplasts. Plasmids containing a full length lentil lipoxygenase cDNA proved to be the most effective, reducing the activity of the target enzyme to 70% of the control value. On the other hand, the full length lentil lipoxygenase cDNA in the sense orientation yielded a 20% increase of lipoxygenase activity.

Original languageEnglish
Pages (from-to)1-3
Number of pages3
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume1259
Issue number1
DOIs
Publication statusPublished - Oct 26 1995

Fingerprint

Lens Plant
Antisense RNA
Lipoxygenase
Protoplasts
Complementary DNA
Plasmids
Genes
Enzymes

Keywords

  • Antisense RNA
  • Electroporation
  • Lentil (Lens culinaris)
  • Lipoxygenase
  • Protoplast

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology

Cite this

Inhibition of lipoxygenase in lentil protoplasts by expression of antisense RNA. / Maccarrone, Mauro; Hilbers, Martin P.; Veldink, Gerrit A.; Vliegenthart, Johannes F G; Agrò, Alessandro Finazzi.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 1259, No. 1, 26.10.1995, p. 1-3.

Research output: Contribution to journal › Article

Maccarrone, M, Hilbers, MP, Veldink, GA, Vliegenthart, JFG & Agrò, AF 1995, 'Inhibition of lipoxygenase in lentil protoplasts by expression of antisense RNA', Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, vol. 1259, no. 1, pp. 1-3. https://doi.org/10.1016/0005-2760(95)00153-4
Maccarrone M, Hilbers MP, Veldink GA, Vliegenthart JFG, Agrò AF. Inhibition of lipoxygenase in lentil protoplasts by expression of antisense RNA. Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1995 Oct 26;1259(1):1-3. https://doi.org/10.1016/0005-2760(95)00153-4
Maccarrone, Mauro ; Hilbers, Martin P. ; Veldink, Gerrit A. ; Vliegenthart, Johannes F G ; Agrò, Alessandro Finazzi. / Inhibition of lipoxygenase in lentil protoplasts by expression of antisense RNA. In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1995 ; Vol. 1259, No. 1. pp. 1-3.
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