Inhibition of lung colonisation of a mouse mammary carcinoma by therapeutic vaccination with interferon-α gene-transduced tumor cells

Ilaria Rossi, Giordano Nicoletti, Lorena Landuzzi, Flavia Frabetti, Carla De Giovanni, Patrizia Nanni, Piero Musiani, Maria Ferrantini, Filippo Belardelli, Pier Luigi Lollini

Research output: Contribution to journalArticle

Abstract

A spontaneously metastatic murine mammary adenocarcinoma, TSA, has been transduced with the gene for interferon α1 (IFN-α). Transfectants were used for the immunotherapy of mice bearing lung colonies induced by the intravenous inoculation of non-transduced parental cells. A significant reduction in the number of tumor colonies was obtained when repeated subcutaneous administrations of mitomycin C-blocked transfectant cells were given, commencing 3 days after an intravenous challenge with TSA cells. Intraperitoneal vaccination induced a stronger anti-tumor response than subcutaneous vaccination, and the proportion of tumor-free mice reached 50%. The potency of IFN-α transfectants was similar to that of IFN-γ transfectants previously obtained from TSA. Admixture of IFN-α and IFN-γ transfectant cells in the same vaccine did not increase the curative effect over that of single vaccines. In nude mice vaccination with IFN-α or IFN-γ transfectants did not lead to a reduction in the number of lung colonies, indicating that an intact T cell response was required for the therapeutic effect observed in immunocompetent mice.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalClinical & Experimental Metastasis
Volume16
Issue number2
DOIs
Publication statusPublished - 1998

Keywords

  • Gene therapy
  • Interferon-alpha
  • Interferon-gamma
  • Lung colony assay
  • Mouse mammary carcinoma
  • Vaccination

ASJC Scopus subject areas

  • Cancer Research

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