Inhibition of medulloblastoma tumorigenesis by the antiproliferative and pro-differentiative gene PC3

Stefano Farioli-Vecchioli, Mirella Tanori, Laura Micheli, Mariateresa Mancuso, Luca Leonardi, Anna Saran, Maria Teresa Ciotti, Elisabetta Ferretti, Alberto Gulino, Simonetta Pazzaglia, Felice Tirone

Research output: Contribution to journalArticlepeer-review

Abstract

Medulloblastoma, the most common brain tumor in childhood, appears to originate from cerebellar granule cell precursors (GCPs), located in the external granular layer (EGL) of the cerebellum. The antiproliferative gene PC3 (Tis21/BTG2) promotes cerebellar neurogenesis by inducing GCPs to shift from proliferation to differentiation. To assess whether PC3 can prevent the neoplastic transformation of GCPs and medulloblastoma development, we crossed transgenic mice conditionally expressing PC3 (TgPC3) in GCPs with Patched1 heterozygous mice (Ptc +/-), a model of medulloblastoma pathogenesis characterized by hyperactivation of the Sonic Hedgehog pathway. Perinatal up-regulation of PC3 in Ptc +/-/TgPC3 mice results in a decrease of medulloblastoma incidence of ∼40% and in a marked reduction of preneoplastic abnormalities, such as hyperplastic EGL areas and lesions. Moreover, overexpression of cyclin D1, hyperproliferation, and defective differentiation - observed in Ptc +/- GCPs - are restored to normality in Ptc +/-/TgPC3 mice. The PC3-mediated inhibition of cyclin D1 expression correlates with recruitment of PC3 to the cyclin D1 promoter, which is accompanied by histone deacetylation. Remarkably, down-regulation of PC3 is observed in preneoplastic lesions, as well as in human and murine medulloblastomas. As a whole, this indicates that PC3 may prevent medulloblastoma development by controlling cell cycle and promoting differentiation of GCPs.

Original languageEnglish
Pages (from-to)2215-2225
Number of pages11
JournalFASEB Journal
Volume21
Issue number9
DOIs
Publication statusPublished - Jul 2007

Keywords

  • Cyclin D1
  • Math1
  • Neurogenesis
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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