TY - JOUR
T1 - Inhibition of monocyte chemotaxis to C-C chemokines by antisense oligonucleotide for cytosolic phospholipase A 2
AU - Locati, Massimo
AU - Lamorte, Giuseppe
AU - Luini, Walter
AU - Introna, Martino
AU - Bernasconi, Sergio
AU - Mantovani, Alberto
AU - Sozzani, Silvano
PY - 1996/3/15
Y1 - 1996/3/15
N2 - Monocyte chemotactic protein (MCP)-1, a member of the C-C (or β) branch of the chemokine superfamily, at chemotactic concentrations, induced a rapid release of [ 3H]arachidonic acid but not of [ 14C]oleic acid from prelabeled human monocytes. This effect was associated with an increase in the intensity of the immunoreactive band corresponding to the phosphorylated form of cytosolic phospholipase A 2 (cPLA 2). To address the role of cPLA 2 in the induction of monocyte chemotaxis, cells were treated with a specific antisense oligonucleotide. Monocytes cultured in the presence of 10 μM antisense oligonucleotide for 48 h showed a marked decrease (57 ± 5%; n = 4) of cPLA 2 expression, as evaluated by Western blot analysis and a nearly complete inhibition (81.8 ± 4.2%; n = 3) of [ 3H]arachidonic acid release in MCP-1-stimulated cells. Monocyte chemotaxis in response to MCP-1 also was inhibited in a concentration-dependent manner by cPLA 2 antisense oligonucleotide (IC 50 = 1.9 ± 1.1 μM; n = 3), with complete inhibition observed between 3 and 10 μM. No inhibition of chemotactic response was observed in monocytes treated with a control oligonucleotide. Monocyte migration in response to MCP-3, RANTES (regulated on activation normal T cells expressed and secreted), and MIP-1α/LD78 also was inhibited (>70%) in antisense oligonucleotide-treated cells. On the contrary, the chemotactic response elicited by formyl-methionyl-leucyl-phenylalanine and C5a, two 'classical' chemotactic agonists, was minimally affected (2 plays a major role in [ 3H]arachidonic acid release by MCP-1 in human monocytes and provide direct evidence for the involvement of cPLA 2 in C-C chemokine-induced monocyte chemotaxis.
AB - Monocyte chemotactic protein (MCP)-1, a member of the C-C (or β) branch of the chemokine superfamily, at chemotactic concentrations, induced a rapid release of [ 3H]arachidonic acid but not of [ 14C]oleic acid from prelabeled human monocytes. This effect was associated with an increase in the intensity of the immunoreactive band corresponding to the phosphorylated form of cytosolic phospholipase A 2 (cPLA 2). To address the role of cPLA 2 in the induction of monocyte chemotaxis, cells were treated with a specific antisense oligonucleotide. Monocytes cultured in the presence of 10 μM antisense oligonucleotide for 48 h showed a marked decrease (57 ± 5%; n = 4) of cPLA 2 expression, as evaluated by Western blot analysis and a nearly complete inhibition (81.8 ± 4.2%; n = 3) of [ 3H]arachidonic acid release in MCP-1-stimulated cells. Monocyte chemotaxis in response to MCP-1 also was inhibited in a concentration-dependent manner by cPLA 2 antisense oligonucleotide (IC 50 = 1.9 ± 1.1 μM; n = 3), with complete inhibition observed between 3 and 10 μM. No inhibition of chemotactic response was observed in monocytes treated with a control oligonucleotide. Monocyte migration in response to MCP-3, RANTES (regulated on activation normal T cells expressed and secreted), and MIP-1α/LD78 also was inhibited (>70%) in antisense oligonucleotide-treated cells. On the contrary, the chemotactic response elicited by formyl-methionyl-leucyl-phenylalanine and C5a, two 'classical' chemotactic agonists, was minimally affected (2 plays a major role in [ 3H]arachidonic acid release by MCP-1 in human monocytes and provide direct evidence for the involvement of cPLA 2 in C-C chemokine-induced monocyte chemotaxis.
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U2 - 10.1074/jbc.271.11.6010
DO - 10.1074/jbc.271.11.6010
M3 - Article
C2 - 8626384
AN - SCOPUS:0029866745
VL - 271
SP - 6010
EP - 6016
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 11
ER -