Inhibition of monocyte chemotaxis to c-c chemokines by antisense oligonucleotide for cytosolic phospholipase A2 (cPLA2)

S. Sozzani, M. Locati, G. Lamorte, W. Luini, M. Introna, S. Bernasconi, A. Mantovani

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Abstract

Monocyte chemotactic protein (MCP)-1, a prototypic C-C chemokine, at chemotactic concentrations, induced a rapid release of [3H]arachidonic acid(AA), but not of [14C]oleic acid, from prelabeled human monocytes. This effect was paralleled by the phosphorylation of cPLA2. To address the role of cPLA2 in monocyte chemotaxis, cells were treated with a specific antisense oligonucleotide. Monocytes cultured with 10 μM antisense oligonucleotide for 48 h showed a decrease (57±5 %) of cPLA2 expression and a strong inhibition of both [3H]AA release (81.814.2%) and chemotaxis in response to MCP-1 (IC50 = 1.9±1.1 μM). No effect was observed with a mismatched control oligonucleotide. Migration to MCP-3, RANTES and MIP-1a/LD78, but not to fMLP and C5a, also was inhibited in antisense oligonucleotide-treated cells. These data show that cPLA2 is the major effector enzyme responsible for [3H]AA release by MCP-1 and provide direct evidence for a role of cPLA2 in C-C chemokine-induced monocyte chemotaxis. The signaling pathway(s) for AA release in 293 cells cotransfected with cPLA2 and chemokine receptors are under investigations.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number6
Publication statusPublished - 1996

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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