Inhibition of poly(ADP-ribosyl)ation does not prevent lymphocyte entry into the cell cycle

Marina Marini, Giovanna Zunica, Daniela Monti, Andrea Cossarizza, Claudio Ortolani, Claudio Franceschi

Research output: Contribution to journalArticlepeer-review


The enzyme poly(ADP-ribosyl)transferase (ADPRT) becomes activated soon after a mitogenic stimulus is applied to lymphocyte cultures. It has also been reported that ADPRT inhibitors prevent cell proliferation when added to cultures at the same time as the mitogen. While this has been ascribed to the need to seal physiologically present DNA strand breaks before cells enter S phase, the presence of DNA strand breaks in quiescent human lymphocytes has been recently questioned. We demonstrate here that non-toxic concentrations of ADPRT inhibitors do not affect lymphocyte blastization and proliferation, as measured by thymidine incorporation and cytofluorimetry. We therefore suggest that ADPRT activation is required for late functions which are not needed for cell cycle progression.

Original languageEnglish
Pages (from-to)146-150
Number of pages5
JournalFEBS Letters
Issue number1-2
Publication statusPublished - Aug 14 1989


  • Cell cycle
  • Enzyme inhibitor
  • Lymphocyte proliferation
  • NAD ADP-ribosyltransferase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Genetics
  • Structural Biology


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