Abstract
The GG2EE macrophage tumor cell line was previously established by immortalization of C3H/HeJ mouse bone marrow cells with the J2 retrovirus which contains the v-myc and v-raf oncogenes. Studies on the control of GGZEE cell proliferation in vitro have recently been performed. We observed that the combination of 5-25 U/ml recombinant mouse interferon-γ (rmIFN-γ) plus 0.03 - 0.3 μg/ml lipopolysaccharide (LPS) markedly inhibited the proliferation of GG2EE cells (by >95%)in vitro, while either agent alone inhibited only by 2EE cells. The combination of IFN-γ + LPS induced more (6-8 U/ml) ILI release. These results suggested that the inhibition of proliferation of GG2EE cells by IFN-γ + LPS could have been mediated in part by cytokines produced by the cells themselves. rhIL1 α at a concentration of 10 U/ml inhibited GG2EE proliferation by 25-30%, while rmIFN-γ (25 U/ml) + rhIL1 α (10 U/ml) inhibited proliferation by 98%. Thus, 10 U/ml rhIL1 α could completely replace LPS in the LPS + rmIFN-γ combination. Further, the combination of low doses of rhIL1 α (0.1 to 1 U/ml) plus rmTNF α (250 U/ml), which together inhibited proliferation by 2EE cells by 98-99%. These results suggest that cytokines produced by the cells themselves can synergize with rmIFN-γ to inhibit the oncogene-driven proliferation of GG2EE cells.
Original language | English |
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Pages (from-to) | 267-276 |
Number of pages | 10 |
Journal | Biotherapy |
Volume | 4 |
Issue number | 4 |
DOIs | |
Publication status | Published - Dec 1992 |
Keywords
- autocrine
- cytokines
- immortalized
- macrophages
- proliferation
ASJC Scopus subject areas
- Pharmacology