Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells

Giovanni Defazio; Beatrice Nico; Maria Trojano; Domenico Ribatti; Maurizio Giorelli; Felicia Ricchiuti; Davide Martino; Luisa Roncali; Paolo Livrea

doi:10.1016/S0006-8993(00)02127-2

Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells

Giovanni Defazio, Beatrice Nico, Maria Trojano, Domenico Ribatti, Maurizio Giorelli, Felicia Ricchiuti, Davide Martino, Luisa Roncali, Paolo Livrea

IRCCS Giovanni Paolo II

Research output: Contribution to journal › Article › peer-review

Abstract

TNFα (100 U/ml, 24 h) upregulated intercellular adhesion molecule 1 (ICAM1) expression and fluid phase endocytosis (FPE) of horseradish peroxidase on brain microvascular endothelial cell (BMEC) culture. The protein kinase C (PKC) inhibitor staurosporin (0.5-10 nM) antagonized ICAM1 expression and FPE due to TNFα, whereas the protein kinase A inhibitor H89 (0.5-10 nM) did not. These findings indicate that a PKC-dependent mechanism may affect TNFα signalling on different barrier properties of BMECs. Copyright (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	245-248
Number of pages	4
Journal	Brain Research
Volume	863
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(00)02127-2
Publication status	Published - Apr 28 2000

Keywords

Brain microvascular endothelial cell
Fluid phase endocytosis
Intercelluar adhesion molecule 1
Protein kinase C
TNFα

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0006-8993(00)02127-2

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Defazio, G., Nico, B., Trojano, M., Ribatti, D., Giorelli, M., Ricchiuti, F., Martino, D., Roncali, L., & Livrea, P. (2000). Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells. Brain Research, 863(1-2), 245-248. https://doi.org/10.1016/S0006-8993(00)02127-2

Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells. / Defazio, Giovanni; Nico, Beatrice; Trojano, Maria; Ribatti, Domenico; Giorelli, Maurizio; Ricchiuti, Felicia; Martino, Davide; Roncali, Luisa; Livrea, Paolo.

In: Brain Research, Vol. 863, No. 1-2, 28.04.2000, p. 245-248.

Research output: Contribution to journal › Article › peer-review

Defazio, G, Nico, B, Trojano, M, Ribatti, D, Giorelli, M, Ricchiuti, F, Martino, D, Roncali, L & Livrea, P 2000, 'Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells', Brain Research, vol. 863, no. 1-2, pp. 245-248. https://doi.org/10.1016/S0006-8993(00)02127-2

Defazio, Giovanni ; Nico, Beatrice ; Trojano, Maria ; Ribatti, Domenico ; Giorelli, Maurizio ; Ricchiuti, Felicia ; Martino, Davide ; Roncali, Luisa ; Livrea, Paolo. / Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells. In: Brain Research. 2000 ; Vol. 863, No. 1-2. pp. 245-248.

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abstract = "TNFα (100 U/ml, 24 h) upregulated intercellular adhesion molecule 1 (ICAM1) expression and fluid phase endocytosis (FPE) of horseradish peroxidase on brain microvascular endothelial cell (BMEC) culture. The protein kinase C (PKC) inhibitor staurosporin (0.5-10 nM) antagonized ICAM1 expression and FPE due to TNFα, whereas the protein kinase A inhibitor H89 (0.5-10 nM) did not. These findings indicate that a PKC-dependent mechanism may affect TNFα signalling on different barrier properties of BMECs. Copyright (C) 2000 Elsevier Science B.V.",

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AU - Ribatti, Domenico

AU - Giorelli, Maurizio

AU - Ricchiuti, Felicia

AU - Martino, Davide

AU - Roncali, Luisa

AU - Livrea, Paolo

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AB - TNFα (100 U/ml, 24 h) upregulated intercellular adhesion molecule 1 (ICAM1) expression and fluid phase endocytosis (FPE) of horseradish peroxidase on brain microvascular endothelial cell (BMEC) culture. The protein kinase C (PKC) inhibitor staurosporin (0.5-10 nM) antagonized ICAM1 expression and FPE due to TNFα, whereas the protein kinase A inhibitor H89 (0.5-10 nM) did not. These findings indicate that a PKC-dependent mechanism may affect TNFα signalling on different barrier properties of BMECs. Copyright (C) 2000 Elsevier Science B.V.

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Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells

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