Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells

Giovanni Defazio; Beatrice Nico; Maria Trojano; Domenico Ribatti; Maurizio Giorelli; Felicia Ricchiuti; Davide Martino; Luisa Roncali; Paolo Livrea

doi:10.1016/S0006-8993(00)02127-2

Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells

Giovanni Defazio, Beatrice Nico, Maria Trojano, Domenico Ribatti, Maurizio Giorelli, Felicia Ricchiuti, Davide Martino, Luisa Roncali, Paolo Livrea

IRCCS Giovanni Paolo II

Research output: Contribution to journal › Article › peer-review

Abstract

TNFα (100 U/ml, 24 h) upregulated intercellular adhesion molecule 1 (ICAM1) expression and fluid phase endocytosis (FPE) of horseradish peroxidase on brain microvascular endothelial cell (BMEC) culture. The protein kinase C (PKC) inhibitor staurosporin (0.5-10 nM) antagonized ICAM1 expression and FPE due to TNFα, whereas the protein kinase A inhibitor H89 (0.5-10 nM) did not. These findings indicate that a PKC-dependent mechanism may affect TNFα signalling on different barrier properties of BMECs. Copyright (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	245-248
Number of pages	4
Journal	Brain Research
Volume	863
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(00)02127-2
Publication status	Published - Apr 28 2000

Keywords

Brain microvascular endothelial cell
Fluid phase endocytosis
Intercelluar adhesion molecule 1
Protein kinase C
TNFα

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/S0006-8993(00)02127-2

Fingerprint Dive into the research topics of 'Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells'. Together they form a unique fingerprint.

Cite this

Defazio, G., Nico, B., Trojano, M., Ribatti, D., Giorelli, M., Ricchiuti, F., Martino, D., Roncali, L., & Livrea, P. (2000). Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells. Brain Research, 863(1-2), 245-248. https://doi.org/10.1016/S0006-8993(00)02127-2

Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells. / Defazio, Giovanni; Nico, Beatrice; Trojano, Maria; Ribatti, Domenico; Giorelli, Maurizio; Ricchiuti, Felicia; Martino, Davide; Roncali, Luisa; Livrea, Paolo.

In: Brain Research, Vol. 863, No. 1-2, 28.04.2000, p. 245-248.

Research output: Contribution to journal › Article › peer-review

Defazio, G, Nico, B, Trojano, M, Ribatti, D, Giorelli, M, Ricchiuti, F, Martino, D, Roncali, L & Livrea, P 2000, 'Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells', Brain Research, vol. 863, no. 1-2, pp. 245-248. https://doi.org/10.1016/S0006-8993(00)02127-2

Defazio, Giovanni ; Nico, Beatrice ; Trojano, Maria ; Ribatti, Domenico ; Giorelli, Maurizio ; Ricchiuti, Felicia ; Martino, Davide ; Roncali, Luisa ; Livrea, Paolo. / Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells. In: Brain Research. 2000 ; Vol. 863, No. 1-2. pp. 245-248.

@article{f440ee48d6a54ed5aa23c1653e3cab13,

title = "Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells",

abstract = "TNFα (100 U/ml, 24 h) upregulated intercellular adhesion molecule 1 (ICAM1) expression and fluid phase endocytosis (FPE) of horseradish peroxidase on brain microvascular endothelial cell (BMEC) culture. The protein kinase C (PKC) inhibitor staurosporin (0.5-10 nM) antagonized ICAM1 expression and FPE due to TNFα, whereas the protein kinase A inhibitor H89 (0.5-10 nM) did not. These findings indicate that a PKC-dependent mechanism may affect TNFα signalling on different barrier properties of BMECs. Copyright (C) 2000 Elsevier Science B.V.",

keywords = "Brain microvascular endothelial cell, Fluid phase endocytosis, Intercelluar adhesion molecule 1, Protein kinase C, TNFα",

author = "Giovanni Defazio and Beatrice Nico and Maria Trojano and Domenico Ribatti and Maurizio Giorelli and Felicia Ricchiuti and Davide Martino and Luisa Roncali and Paolo Livrea",

year = "2000",

month = apr,

day = "28",

doi = "10.1016/S0006-8993(00)02127-2",

language = "English",

volume = "863",

pages = "245--248",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells

AU - Defazio, Giovanni

AU - Nico, Beatrice

AU - Trojano, Maria

AU - Ribatti, Domenico

AU - Giorelli, Maurizio

AU - Ricchiuti, Felicia

AU - Martino, Davide

AU - Roncali, Luisa

AU - Livrea, Paolo

PY - 2000/4/28

Y1 - 2000/4/28

N2 - TNFα (100 U/ml, 24 h) upregulated intercellular adhesion molecule 1 (ICAM1) expression and fluid phase endocytosis (FPE) of horseradish peroxidase on brain microvascular endothelial cell (BMEC) culture. The protein kinase C (PKC) inhibitor staurosporin (0.5-10 nM) antagonized ICAM1 expression and FPE due to TNFα, whereas the protein kinase A inhibitor H89 (0.5-10 nM) did not. These findings indicate that a PKC-dependent mechanism may affect TNFα signalling on different barrier properties of BMECs. Copyright (C) 2000 Elsevier Science B.V.

AB - TNFα (100 U/ml, 24 h) upregulated intercellular adhesion molecule 1 (ICAM1) expression and fluid phase endocytosis (FPE) of horseradish peroxidase on brain microvascular endothelial cell (BMEC) culture. The protein kinase C (PKC) inhibitor staurosporin (0.5-10 nM) antagonized ICAM1 expression and FPE due to TNFα, whereas the protein kinase A inhibitor H89 (0.5-10 nM) did not. These findings indicate that a PKC-dependent mechanism may affect TNFα signalling on different barrier properties of BMECs. Copyright (C) 2000 Elsevier Science B.V.

KW - Brain microvascular endothelial cell

KW - Fluid phase endocytosis

KW - Intercelluar adhesion molecule 1

KW - Protein kinase C

KW - TNFα

UR - http://www.scopus.com/inward/record.url?scp=0034724969&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034724969&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(00)02127-2

DO - 10.1016/S0006-8993(00)02127-2

M3 - Article

C2 - 10773213

AN - SCOPUS:0034724969

VL - 863

SP - 245

EP - 248

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -

Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this