Inhibition of protein kinase C counteracts TNFα-induced intercellular adhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells

Giovanni Defazio, Beatrice Nico, Maria Trojano, Domenico Ribatti, Maurizio Giorelli, Felicia Ricchiuti, Davide Martino, Luisa Roncali, Paolo Livrea

Research output: Contribution to journalArticlepeer-review

Abstract

TNFα (100 U/ml, 24 h) upregulated intercellular adhesion molecule 1 (ICAM1) expression and fluid phase endocytosis (FPE) of horseradish peroxidase on brain microvascular endothelial cell (BMEC) culture. The protein kinase C (PKC) inhibitor staurosporin (0.5-10 nM) antagonized ICAM1 expression and FPE due to TNFα, whereas the protein kinase A inhibitor H89 (0.5-10 nM) did not. These findings indicate that a PKC-dependent mechanism may affect TNFα signalling on different barrier properties of BMECs. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)245-248
Number of pages4
JournalBrain Research
Volume863
Issue number1-2
DOIs
Publication statusPublished - Apr 28 2000

Keywords

  • Brain microvascular endothelial cell
  • Fluid phase endocytosis
  • Intercelluar adhesion molecule 1
  • Protein kinase C
  • TNFα

ASJC Scopus subject areas

  • Neuroscience(all)

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