Abstract
TNFα (100 U/ml, 24 h) upregulated intercellular adhesion molecule 1 (ICAM1) expression and fluid phase endocytosis (FPE) of horseradish peroxidase on brain microvascular endothelial cell (BMEC) culture. The protein kinase C (PKC) inhibitor staurosporin (0.5-10 nM) antagonized ICAM1 expression and FPE due to TNFα, whereas the protein kinase A inhibitor H89 (0.5-10 nM) did not. These findings indicate that a PKC-dependent mechanism may affect TNFα signalling on different barrier properties of BMECs. Copyright (C) 2000 Elsevier Science B.V.
Original language | English |
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Pages (from-to) | 245-248 |
Number of pages | 4 |
Journal | Brain Research |
Volume | 863 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - Apr 28 2000 |
Keywords
- Brain microvascular endothelial cell
- Fluid phase endocytosis
- Intercelluar adhesion molecule 1
- Protein kinase C
- TNFα
ASJC Scopus subject areas
- Neuroscience(all)