Inhibition of rat fibroblast cell proliferation at specific cell cycle stages by cocaine

Paolo Di Francesco, Francesca Pica, Cartesio Favalli, Ezio Tubaro, Enrico Garaci

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have analyzed the role of cocaine in the control of the rat fibroblast (EL2) cell proliferation. Our data show a dose-related effect on the inhibition of DNA synthesis and cell growth when cocaine is added with serum or with a pure growth factor [Epidermal Growth Factor (EGF)]. Pretreatment by drug did not appreciably enhance the inhibition of S-phase entry above that obtained when cocaine and mitogen were added simultaneously. On the contrary, exposure of quiescent EL2 cells to cocaine has little or no effect on DNA synthesis, when drug is removed before the mitogenic stimulus. Moreover, even when cocaine is added after EGF, an exposure only within 1-8 hours is required in order to inhibit stimulation of DNA synthesis. Cocaine also suppressed the general increase in protein synthesis that occurs during the first hour after EGF addition. The combined data suggest that cocaine inhibits the traverse of mitogen-stimulated quiescent EL2 cells from Go to S phase by acting on processes that take place during the initial phase of the cell cycle.

Original languageEnglish
Pages (from-to)549-558
Number of pages10
JournalCell Biology International Reports
Volume14
Issue number6
DOIs
Publication statusPublished - 1990

Fingerprint

Cocaine
Cell Cycle
Fibroblasts
Cell Proliferation
Epidermal Growth Factor
S Phase
Mitogens
DNA
Pharmaceutical Preparations
Intercellular Signaling Peptides and Proteins
Growth
Serum
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Inhibition of rat fibroblast cell proliferation at specific cell cycle stages by cocaine. / Di Francesco, Paolo; Pica, Francesca; Favalli, Cartesio; Tubaro, Ezio; Garaci, Enrico.

In: Cell Biology International Reports, Vol. 14, No. 6, 1990, p. 549-558.

Research output: Contribution to journalArticle

Di Francesco, Paolo ; Pica, Francesca ; Favalli, Cartesio ; Tubaro, Ezio ; Garaci, Enrico. / Inhibition of rat fibroblast cell proliferation at specific cell cycle stages by cocaine. In: Cell Biology International Reports. 1990 ; Vol. 14, No. 6. pp. 549-558.
@article{9a6755a7e3a84912bf9fd8d8b6feb6a0,
title = "Inhibition of rat fibroblast cell proliferation at specific cell cycle stages by cocaine",
abstract = "We have analyzed the role of cocaine in the control of the rat fibroblast (EL2) cell proliferation. Our data show a dose-related effect on the inhibition of DNA synthesis and cell growth when cocaine is added with serum or with a pure growth factor [Epidermal Growth Factor (EGF)]. Pretreatment by drug did not appreciably enhance the inhibition of S-phase entry above that obtained when cocaine and mitogen were added simultaneously. On the contrary, exposure of quiescent EL2 cells to cocaine has little or no effect on DNA synthesis, when drug is removed before the mitogenic stimulus. Moreover, even when cocaine is added after EGF, an exposure only within 1-8 hours is required in order to inhibit stimulation of DNA synthesis. Cocaine also suppressed the general increase in protein synthesis that occurs during the first hour after EGF addition. The combined data suggest that cocaine inhibits the traverse of mitogen-stimulated quiescent EL2 cells from Go to S phase by acting on processes that take place during the initial phase of the cell cycle.",
author = "{Di Francesco}, Paolo and Francesca Pica and Cartesio Favalli and Ezio Tubaro and Enrico Garaci",
year = "1990",
doi = "10.1016/0309-1651(90)91182-4",
language = "English",
volume = "14",
pages = "549--558",
journal = "Cell Biology International Reports",
issn = "0309-1651",
publisher = "Academic Press Inc.",
number = "6",

}

TY - JOUR

T1 - Inhibition of rat fibroblast cell proliferation at specific cell cycle stages by cocaine

AU - Di Francesco, Paolo

AU - Pica, Francesca

AU - Favalli, Cartesio

AU - Tubaro, Ezio

AU - Garaci, Enrico

PY - 1990

Y1 - 1990

N2 - We have analyzed the role of cocaine in the control of the rat fibroblast (EL2) cell proliferation. Our data show a dose-related effect on the inhibition of DNA synthesis and cell growth when cocaine is added with serum or with a pure growth factor [Epidermal Growth Factor (EGF)]. Pretreatment by drug did not appreciably enhance the inhibition of S-phase entry above that obtained when cocaine and mitogen were added simultaneously. On the contrary, exposure of quiescent EL2 cells to cocaine has little or no effect on DNA synthesis, when drug is removed before the mitogenic stimulus. Moreover, even when cocaine is added after EGF, an exposure only within 1-8 hours is required in order to inhibit stimulation of DNA synthesis. Cocaine also suppressed the general increase in protein synthesis that occurs during the first hour after EGF addition. The combined data suggest that cocaine inhibits the traverse of mitogen-stimulated quiescent EL2 cells from Go to S phase by acting on processes that take place during the initial phase of the cell cycle.

AB - We have analyzed the role of cocaine in the control of the rat fibroblast (EL2) cell proliferation. Our data show a dose-related effect on the inhibition of DNA synthesis and cell growth when cocaine is added with serum or with a pure growth factor [Epidermal Growth Factor (EGF)]. Pretreatment by drug did not appreciably enhance the inhibition of S-phase entry above that obtained when cocaine and mitogen were added simultaneously. On the contrary, exposure of quiescent EL2 cells to cocaine has little or no effect on DNA synthesis, when drug is removed before the mitogenic stimulus. Moreover, even when cocaine is added after EGF, an exposure only within 1-8 hours is required in order to inhibit stimulation of DNA synthesis. Cocaine also suppressed the general increase in protein synthesis that occurs during the first hour after EGF addition. The combined data suggest that cocaine inhibits the traverse of mitogen-stimulated quiescent EL2 cells from Go to S phase by acting on processes that take place during the initial phase of the cell cycle.

UR - http://www.scopus.com/inward/record.url?scp=0025371520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025371520&partnerID=8YFLogxK

U2 - 10.1016/0309-1651(90)91182-4

DO - 10.1016/0309-1651(90)91182-4

M3 - Article

C2 - 2369763

AN - SCOPUS:0025371520

VL - 14

SP - 549

EP - 558

JO - Cell Biology International Reports

JF - Cell Biology International Reports

SN - 0309-1651

IS - 6

ER -