Inhibition of receptor-dependent urokinase signaling by specific ser to glu substitutions

Maria Vincenza Carriero, Paola Franco, Lucia Gargiulo, Immacolata Vocca, Letizia Cito, Laura Fontana, Ciro Iaccarino, Giovanna Del Pozzo, John Guardiola, Maria Patrizia Stopelli

Research output: Contribution to journalArticlepeer-review

Abstract

We have previously reported that phosphorylation of human urokinase on Ser138/303 abolishes its catalytic-independent motogen and proadhesive abilities, whereas receptor binding is not affected. Here we show that substitution of the two relevant serines with glutamic acid residues impairs the ability of urokinase to mobilize a variety of human and mouse cell lines as well as human primary T lymphocytes. Accordingly, urokinase receptor-dependent signaling, leading to cytoskeletal rearrangements and paxillin re-distribution, does not occur in MCF-7 breast carcinoma cells exposed to 'phosphorylation-like' urokinase. Unlike the wild-type form, di-substituted urokinase is unable to induce the physical association of urokinase receptor with αvβ5 vitronectin receptor, which is required for MCF-7 urokinase-dependent cell migration. Finally, the di-substituted variant fails to activate p55fgr, a member of the Src tyrosine kinase family, which mediates cell migration and adhesion of U937 myelomonocytic cells. In conclusion, the finding that specific amino acid substitutions strongly interfere with the ability of urokinase to stimulate cell migration, and the associated intracellular events uncover a novel way to regulate urokinase receptor-dependent signaling.

Original languageEnglish
Pages (from-to)107-113
Number of pages7
JournalBiological Chemistry
Volume383
Issue number1
DOIs
Publication statusPublished - 2002

Keywords

  • Antagonist
  • Cell migration
  • Serine phosphorylation
  • Urokinase receptor

ASJC Scopus subject areas

  • Biochemistry

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