Inhibition of replication of virus transformed fibroblasts by antibodies to RNA

L. Varesio, P. Cappuccinelli, G. Forni

Research output: Contribution to journalArticlepeer-review

Abstract

The activity of 7S immunoglobulin (Ig) antibody to RNA, obtained in rabbits after a prolonged immunization with RNA methylated bovine serum albumin complex, was evaluated in vitro on normal (3T3) and simian virus 40 transformed (SV 3T3) mouse fibroblasts. The presence of anti RNA antibody in the culture medium inhibited both the SV 3T3 cell proliferation and the [3H]thymidine incorporation. In contrast, an increased [3H]uridine incorporation was evident within 48 and 96 hr of culture. No significant modification in these 3 parameters was observed in 3T3 cultures treated in the same manner. Both 3T3 and SV 3T3 showed cytoplasmic fluorescence when cultured in the presence of fluoresceinated anti RNA Ig. However, with the indirect fluorescence technique anti RNA Ig were detected in SV 3T3 cytoplasm only. These data suggest that anti RNA Ig were taken up by both 3T3 and SV 3T3, but only in SV 3T3 did the anti RNA Ig retain their antigenic properties and block cellular proliferation.

Original languageEnglish
Pages (from-to)3558-3565
Number of pages8
JournalCancer Research
Volume35
Issue number12
Publication statusPublished - 1975

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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